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Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria
The latest progress on the biochemistry and genetics of the energy metabolism of model acetogens are discussed, elucidating how these bacteria couple CO2 fixation to energy conservation. Expand
Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotes
The recent data that unraveled a novel ion-motive electron transport chain, the Rnf complex, that energetically couples the cellular ferredoxin to the pyridine nucleotide pool is summarized. Expand
Osmoadaptation in bacteria and archaea: common principles and differences.
The temporal sequence of events after an osmotic upshock will be discussed, with a focus on the most rapid response, notably the mechanisms of transport activation at the protein level, and different signals for osmolality will be compared. Expand
Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase
  • E. Biegel, V. Müller
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 4 October 2010
Results from inhibitor studies are consistent with the hypothesis that Fno activity coupled to Na+ translocation is catalyzed by the Rnf complex, a membrane-bound, iron–sulfur and flavin-containing electron transport complex encoded by many bacterial and some archaeal genomes. Expand
A novel mode of lactate metabolism in strictly anaerobic bacteria.
The lactate dehydrogenase (LDH) is purified from the strictly anaerobic, acetogenic bacterium Acetobacterium woodii, which uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD(+) as oxidant. Expand
Energy Conservation in Acetogenic Bacteria
  • V. Müller
  • Biology, Medicine
  • Applied and Environmental Microbiology
  • 1 November 2003
The anaerobic way of life comprises a great metabolic diversity and, correspondingly, diversity of energy-generating mechanisms ([52][1]). The facultative anaerobes have a respiratory system thatExpand
Bioenergetic constraints for conversion of syngas to biofuels in acetogenic bacteria
This review discusses the bioenergetic constraints for the conversion of syngas to different biofuels and focuses on Acetobacterium woodii, which is the best understood acetogen in terms of energy conservation. Expand
An Ancient Pathway Combining Carbon Dioxide Fixation with the Generation and Utilization of a Sodium Ion Gradient for ATP Synthesis
The genome sequence of A. woodii solves the enigma and uncovers Rnf as the only ion-motive enzyme coupled to the pathway and unravels a metabolism designed to produce reduced ferredoxin and overcome energetic barriers by virtue of electron-bifurcating, soluble enzymes. Expand
Salinity-Dependent Switching of Osmolyte Strategies in a Moderately Halophilic Bacterium: Glutamate Induces Proline Biosynthesis in Halobacillus halophilus
It is demonstrated that the solute glutamate is involved in the switch of osmolyte strategy from glutamate to proline as the dominant compatible solute during the transition from moderate to high salinity. Expand
A Bacterial Electron-bifurcating Hydrogenase*
Spectroscopic analyses revealed that NAD+ and ferredoxin reduction are strictly coupled and that they are reduced in a 1:1 stoichiometry. Expand