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Activation of the Cooh-Terminal Src Kinase (Csk) by Camp-Dependent Protein Kinase Inhibits Signaling through the T Cell Receptor
A mechanism whereby PKA through activation of Csk intersects signaling by Src kinases and inhibits T cell activation is proposed, which leads to a two- to fourfold increase in Csk activity that is necessary for cAMP-mediated inhibition of TCR-induced interleukin 2 secretion. Expand
Structure, function, and regulation of human cAMP-dependent protein kinases.
The existence of a number of anchoring proteins specific to either RIIalpha or RIIbeta, and which localize cAKII isozymes toward distinct substrates at defined subcellular loci, strongly supports the idea that specific functions can be assigned to the various cAK isoz enzymes. Expand
Localization of a novel human A-kinase-anchoring protein, hAKAP220, during spermatogenesis.
Using a combination of protein kinase A type II overlay screening, rapid amplification of cDNA ends, and database searches, a contig of 9923 bp was assembled and characterized in which the openExpand
Steroid binding in polyacrylamide gels. Quantitation at steady state conditions.
Using steady state gel electrophoresis of nonlabeled samples and samples saturated with labeled steroid, concentrations of testicular androgen-binding protein in rat and rabbit, as well as concentration of rabbit, monkey, and human serum testosterone-binding globulin have been measured. Expand
Location of cAMP-dependent protein kinase type I with the TCR-CD3 complex.
Immunocytochemistry and immunoprecipitation studies of the molecular mechanism by which cAKI inhibits T CR-CD3-dependent T cell replication demonstrated that regulatory subunit I alpha, along with its associated kinase activity, translocated to and interacted with the TCR- CD3 complex during T cell activation and capping. Expand
Cyclic AMP-dependent protein kinase type I mediates the inhibitory effects of 3',5'-cyclic adenosine monophosphate on cell replication in human T lymphocytes.
It is concluded that activation of cAKI is sufficient to inhibit T lymphocyte proliferation and the membrane-bound cAKII may mediate cAMP actions not related to cell replication. Expand
Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase.
The molecular cloning of a third isoform of C, from a human testis cDNA library, as well as the isolation of human cDNAs for C alpha and C beta are reported, suggesting that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits. Expand
Novel isozymes of cAMP-dependent protein kinase exist in human cells due to formation of RI alpha-RI beta heterodimeric complexes.
RI alpha-RI beta heterodimers complexed with the catalytic subunit represent a novel isozyme of cAKI (RI alpha RI beta C2), which enhances the possibilities for diversification of cAMP-mediated effects. Expand
Molecular cloning, cDNA structure and deduced amino acid sequence for a type I regulatory subunit of cAMP-dependent protein kinase from human testis.
A 1.5 kilobase cDNA clone containing the entire coding region for a regulatory subunit of type I cAMP-dependent protein kinase (RI) was isolated from a human testis cDNA library and shows 98% and 97% homology to the bovine skeletal muscle RI and rat brain RI, respectively. Expand
Subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase show differential and distinct expression patterns during germ cell differentiation: alternative polyadenylation in germ
The present study demonstrates the presence of unique smaller-sized mRNAs in germ cells compared with somatic cells, and suggests the selection of shorter mRNA species, with higher stability, may be essential for the delayed translation observed in spermatids. Expand