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Revision of Begomovirus taxonomy based on pairwise sequence comparisons
- Judith K. Brown, F. Zerbini, +12 authors A. Varsani
- Biology, Medicine
- Archives of Virology
- 18 April 2015
Revised guidelines for the classification and nomenclature of begomoviruses are proposed and genome-wide pairwise identities of 91 % and 94 % are proposed as the demarcation threshold for begomviruses belonging to different species and strains, respectively. Expand
Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite begomovirus.
SLCMV DNA A has biological characteristics of a monopartite begomovirus, and the virus probably evolved by acquisition of a DNA B component from ICMV, which was transmitted with low efficiency from cassava to Nicotiana benthamiana. Expand
Emerging geminivirus problems: A serious threat to crop production
Human activity has also played an important role in the emergence of serious geminivirus diseases across the globe, like the changes in cropping systems, the introduction of new crops, the movement of infected planting materials and the introducing of host susceptibility genes through the exchange of germplasm. Expand
A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)
A user-friendly computer program is presented, SDT (species demarcation tool), for calculating and graphically displaying pairwise genome identity scores, and modified versions of these protocols are proposed that should substantially minimise the degree of classification inconsistency under the current system. Expand
Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B…
- P. Jyothsna, Q. M. Haq, +5 authors V. G. Malathi
- Biology, Medicine
- Applied Microbiology and Biotechnology
- 10 January 2013
Inoculation with the cloned component DNA A and DNA B were agroinoculated on Nicotiana benthamiana and tomato plants with or without betasatellites, CLCuMuB or LuLDB. Expand
Systemic infection of Vigna mungo using the cloned DNAs of the blackgram isolate of mungbean yellow mosaic geminivirus through agroinoculation and transmission of the progeny virus by whiteflies.
It has been shown that MYMV has a bipartite genome and Tandem dimeric constructs of DNA-A and DNA-B were infectious when inoculated together and induced systemic yellow mosaic symptoms. Expand
Genetic engineering for virus resistance
T tremendous advances have taken place in the understanding of plant–virus interaction in the process of pathogenesis and resistance, which has opened up the possibility of an entirely new approach of genetic engineering towards controlling plant virus diseases. Expand
Severe leaf curl disease of cowpea – a new disease of cowpea in northern India caused by Mungbean yellow mosaic India virus and a satellite DNA β
Potato leaf curl – a new disease of potato in northern India caused by a strain of Tomato leaf curl New Delhi virus
The nucleotide sequence data indicate that the cause of the leaf curl disease in potato is a virus closely related to ToLCNDV which, unlike the strain infecting potato, is not sap-transmissible and is not known to infect potato naturally. Expand
An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legumes.
- A. Rouhibakhsh, J. Priya, M. Periasamy, Q. M. Haq., V. G. Malathi
- Biology, Medicine
- Journal of virological methods
PCR amplifiable DNA could be extracted from mature leaves of legume hosts rich in polyphenols, tannins and polysaccharides and the system is robust and the protocol is useful for the detection and identification of begomoviruses infecting grain legumes. Expand