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Differentiating the functional role of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha (EPAS-1) by the use of RNA interference: erythropoietin is a HIF-2alpha target gene in Hep3B and Kelly
TLDR
A remarkably restricted target gene specificity of the HIFalpha subunits is found, which can be overcome in cells with perturbations in the pVHL/HIF system and under forced expression.
Design of antisense oligonucleotides stabilized by locked nucleic acids.
TLDR
These chimeric LNA/DNA oligonucleotides are more stable than isosequential phosphorothioates and 2'-O-methyl gapmers, which have half-lives of 10 and 12 h, respectively.
Differentiating the functional role of hypoxia‐inducible factor (HIF)‐1α and HIF‐2α (EPAS‐1) by the use of RNA interference: erythropoietin is a HIF‐2α target gene in Hep3B and Kelly cells
TLDR
A remarkably restricted target gene specificity of the HIFα subunits is found, which can be overcome in cells with perturbations in the pVHL/HIF system and under forced expression.
Probing the SELEX Process with Next-Generation Sequencing
TLDR
A new selection scheme is suggested that avoids a high number of iterative selection rounds while reducing time, PCR bias, and artifacts and high affinity aptamers can be readily identified simply by copy number enrichment in the first selection rounds.
Evaluating the Risk of Patent Infringement by Means of Semantic Patent Analysis: The Case of DNA Chips
TLDR
A method for detecting the risks of patent infringement by evaluating similarities between patent documents on the basis of semantic patent analysis is presented, which enables the user to visualize similarities in the contents on a semantic patent map by means of multi-dimensional scaling.
RNA cleaving '10-23' DNAzymes with enhanced stability and activity.
TLDR
This study optimises the design of a DNAzyme targeting the 5'-non-translated region of the human rhinovirus 14, a common cold virus, with regard to its kinetic properties and its stability against nucleases, yielding a species with up to 10-fold enhanced activity and significantly elevated stability againstucleolytic cleavage.
Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2'-O-methyl RNA, phosphorothioates and small interfering RNA.
TLDR
An LNA gapmer was found to be the most efficient single-stranded antisense oligonucleotide, with an IC50 of 0.4 nM being 175-fold lower than that of commonly used phosphorothioates.
Mirror-design of L-oligonucleotide ligands binding to L-arginine
TLDR
The mirror-design of a stable 38-mer L-oligoribonucleotide ligand that binds to L-arginine is described that was able to bind to a short peptide containing the basic region of the human immunodeficiency virus type-1 Tat-protein.
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