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The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
It is demonstrated that by this method mutants can be constructed with marker yields in excess of 70% and a rigorous selection can be applied for phage carrying the markers of the (-) strand. Expand
AFM study of Escherichia coli RNA polymerase σ⁷⁰ subunit aggregation.
Atomic force microscopy data, Congo red binding assay, and sodium dodecyl sulfate gel electrophoresis confirm the amyloid nature of observed aggregates, and Polymerization of σ⁷⁰ subunit may be a competitive process with its three-dimensional crystallization and association with core RNA polymerase. Expand
DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp).
It is demonstrated that in conditions when the concatemeric duplex is stable a water-soluble carbodiimide induces efficient polymerization of the 3'- or 5'-phosphorylated decanucleotide, and the resulting polymers d(TGGCCAAGCTp)2-10 contain only natural phosphodiester bonds. Expand
‘Half‐site’ affinity modification of tryptophanyl‐tRNA synthetase leads to ‘freezing’ of the free subunit
The modified enzyme was used to evaluate the interactions between two subunits in the dimeric enzyme protein, and the ‘halfsite’ phosphorylation by an AMP residue from mesitoyl-[14C]AMP is observed. Expand
An active-site-directed adenosine triphosphate analogue binds to the beta-subunits of factor F1 mitochondrial adenosine triphosphatase with its triphosphate moiety.
The results suggest that ATP may also bind to the beta-subunit of the adenosine triph phosphatase with its triphosphate moiety. Expand
Investigation of activation of phosphate groups in mono- and oligonucleotides with mesitoyl chloride
It has been demonstrated with the use of 31P NMR pulsed spectroscopy that the reaction of mesitoyl chloride (MsCOCl) both with terminal and internucleotide phosphate groups pA, d(MeOTr)TpT and dpTpTExpand
Is transketolase-like protein, TKTL1, transketolase?
It is demonstrated here that DhTKT is devoid of transketolase activity and lacks bound thiamine diphosphate (ThDP). Expand
[Introduction of tri- and tetraphosphate internucleotide bonds into oligodeoxyribonucleotides].
Dodecadeoxyribonucleotides d(AGCTTGpppGCTGCA) and d(AGCTTGppppGCTGCA) were obtained by template directed chemical condensation induced by a water-soluble carbodiimide.
Isolation and site-directed mutagenesis of DNA methyltransferase SssI
To verify the role of Ser300 in recognizing the CpG sequence by the enzyme, Ser300 was replaced by Gly or Pro and the substitution had virtually no effect on DNA binding and methylation by M.SssI apart from a slight decrease in binding in the case of S300P. Expand