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Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.
Obtaining complete protein inventories for subcellular regions is a challenge that often limits our understanding of cellular function, especially for regions that are impossible to purify and areExpand
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Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio
Taking control of cellular NAD+ concentrations Cellular concentrations of the nicotinamide adenine dinucleotide (NAD+) are critical for proper metabolism and are often altered in aging and disease.Expand
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IGF2BP2/IMP2-Deficient mice resist obesity through enhanced translation of Ucp1 mRNA and Other mRNAs encoding mitochondrial proteins.
Although variants in the IGF2BP2/IMP2 gene confer risk for type 2 diabetes, IMP2, an RNA binding protein, is not known to regulate metabolism. Imp2(-/-) mice gain less lean mass after weaning andExpand
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Meclizine Inhibits Mitochondrial Respiration through Direct Targeting of Cytosolic Phosphoethanolamine Metabolism*
Background: Previous studies have shown that meclizine inhibits respiration in intact cells, but not in isolated mitochondria, via an unknown mechanism. Results: Meclizine directly inhibits PCYT2Expand
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IcmF Is a Fusion between the Radical B12 Enzyme Isobutyryl-CoA Mutase and Its G-protein Chaperone*
Coenzyme B12 is used by two highly similar radical enzymes, which catalyze carbon skeleton rearrangements, methylmalonyl-CoA mutase and isobutyryl-CoA mutase (ICM). ICM catalyzes the reversibleExpand
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Novel Coenzyme B12-dependent Interconversion of Isovaleryl-CoA and Pivalyl-CoA*
Background: IcmF is a fusion between a coenzyme B12-dependent isobutyryl-CoA/n-butyryl-CoA isomerase and a G-protein chaperone. Results: IcmF also isomerizes isovaleryl-CoA to pivalyl-CoA and isExpand
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A genetically encoded tool for manipulation of NADP+/NADPH in living cells
NADH and NADPH are redox coenzymes broadly required for energy metabolism, biosynthesis and detoxification. Despite detailed knowledge of specific enzymes and pathways that utilize these coenzymes, aExpand
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Visualization of a radical B12 enzyme with its G-protein chaperone
Significance Metalloproteins are ubiquitous, accounting for about 30–50% of all proteins. Their functions are wide-ranging, but metalloproteins are frequently used to carry out challenging molecularExpand
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Engineered and Native Coenzyme B12-dependent Isovaleryl-CoA/Pivalyl-CoA Mutase*
Background: IcmF exhibits low isovaleryl-CoA/pivalyl-CoA mutase (PCM) activity. Results: IcmF mutants designed to enhance PCM activity were susceptible to inactivation prompting a bioinformaticsExpand
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Novel B(12)-dependent acyl-CoA mutases and their biotechnological potential.
The recent spate of discoveries of novel acyl-CoA mutases has engendered a growing appreciation for the diversity of 5'-deoxyadenosylcobalamin-dependent rearrangement reactions. The prototype of theExpand
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