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An alternative route to cyclic terpenes by reductive cyclization in iridoid biosynthesis
TLDR
The discovery of iridoid synthase is reported, a plant-derived enzyme that generates the iridoids ring scaffold, as evidenced by biochemical assays, gene silencing, co-expression analysis and localization studies, and the prospects of using unrelated reductases to generate artificial cyclic scaffolds are highlighted.
De novo production of the plant-derived alkaloid strictosidine in yeast
TLDR
It is demonstrated how strictosidine can be produced de novo in a Saccharomyces cerevisiae host from 14 known monoterpene indole alkaloid pathway genes, along with an additional seven genes and three gene deletions that enhance secondary metabolism.
Emerging and Emerged Pathogenic Candida Species: Beyond the Candida albicans Paradigm
TLDR
Although Candida albicans remains the most frequently isolated agent of candidiasis, non-albicans Candida (NAC) species now account for a substantial part of clinical isolates collected worldwide in hospitals, and a complementary set of about 20 opportunistic NAC species is also known, but exhibits lower isolation rates.
Strictosidine activation in Apocynaceae: towards a "nuclear time bomb"?
TLDR
A spatial model was drawn to explain the role of the subcellular sequestration of STR and SGD to control the MIA metabolic flux under normal physiological conditions and illustrates the possible mechanism of massive activation of the strictosidine vacuolar pool upon enzyme-substrate reunion occurring during potential herbivore feeding.
The subcellular organization of strictosidine biosynthesis in Catharanthus roseus epidermis highlights several trans‐tonoplast translocations of intermediate metabolites
TLDR
The spatial characterization of this mechanism was carried out by a cellular and subcellular study of three enzymes catalyzing the synthesis of the two strictosidine precursors (i.e. tryptamine and secologanin), demonstrating the importance of trans‐tonoplast translocation events during these metabolic processes.
A single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in Catharanthus roseus
TLDR
The cloning and functional validation of an IDI encoding cDNA (CrIDI1) from Catharanthus roseus that produces high amount of monoterpenoid indole alkaloids is reported and it is established that this complex plastid/mitochondria/peroxisomes triple targeting occurring in C. roseus producing specialized isoprenoid secondary metabolites is somehow different from the situation observed in A. thaliana mainly producing housekeeping isopranoid metabolites.
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