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The 2-Oxoacid Dehydrogenase Complexes in Mitochondria Can Produce Superoxide/Hydrogen Peroxide at Much Higher Rates Than Complex I*
Background: At the redox potential of NADH/NAD+, at least four mitochondrial sites produce superoxide/H2O2. Results: We compare their capacities in situ in isolated mitochondria. Conclusion: MaximumExpand
Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species.
Self-regulation of the 2-oxo acid dehydrogenase complexes during catalysis was studied. Radical species as side products of catalysis were detected by spin trapping, lucigenin fluorescence andExpand
2-Oxo acid dehydrogenase complexes in redox regulation.
  • V. Bunik
  • Biology, Medicine
  • European journal of biochemistry
  • 1 March 2003
A number of cellular systems cooperate in redox regulation, providing metabolic responses according to changes in the oxidation (or reduction) of the redox active components of a cell. Key systems ofExpand
Metabolic control exerted by the 2-oxoglutarate dehydrogenase reaction: a cross-kingdom comparison of the crossroad between energy production and nitrogen assimilation.
Mechanism-based inhibitors and both forward and reverse genetics have proved to be essential tools in revealing roles for specific enzymatic processes in cellular function. Here, we reviewExpand
Structure–function relationships in the 2‐oxo acid dehydrogenase family: Substrate‐specific signatures and functional predictions for the 2‐oxoglutarate dehydrogenase‐like proteins
Structural relationship within the family of the thiamine diphosphate‐dependent 2‐oxo acid dehydrogenases was analyzed by combining different methods of sequence alignment with crystallographic andExpand
Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis
Thiamin (vitamin B1) is a pharmacological agent boosting central metabolism through the action of the coenzyme thiamin diphosphate (ThDP). However, positive effects, including improved cognition, ofExpand
Inhibition of 2-Oxoglutarate Dehydrogenase in Potato Tuber Suggests the Enzyme Is Limiting for Respiration and Confirms Its Importance in Nitrogen Assimilation1[W][OA]
The 2-oxoglutarate dehydrogenase complex constitutes a mitochondrially localized tricarboxylic acid cycle multienzyme system responsible for the conversion of 2-oxoglutarate to succinyl-coenzyme AExpand
Novel isoenzyme of 2‐oxoglutarate dehydrogenase is identified in brain, but not in heart
2‐Oxoglutarate dehydrogenase (OGDH) is the first and rate‐limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neurodegeneration. The essential role of thisExpand
The α-ketoglutarate-dehydrogenase complex
Damage from oxidative stress and mitochondrial dysfunction occur together in many common neurodegenerative diseases. The enzymes that form the mitochondrial α-ketoglutarate-dehydrogenase complexExpand
Kinetic properties of the 2-oxoglutarate dehydrogenase complex from Azotobacter vinelandii evidence for the formation of a precatalytic complex with 2-oxoglutarate.
The 2-oxoglutarate dehydrogenase complex was purified from Azotobacter vinelandii. The complex consists of three components, 2-oxoglutarate dehydrogenase/decarboxylase (E1o), lipoateExpand