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Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro
TLDR
Functional similarities between the replication and checkpoint clamp loader/DNA clamp pairs are demonstrated and Electron microscopic analyses of the reaction products indicate that the 9-1-1 ring is clamped around the DNA.
Studies with the Human Cohesin Establishment Factor, ChlR1
TLDR
Findings are consistent with a role of hChlR1 in the establishment of sister chromatid cohesion and suggest that its action may contribute to lagging strand processing events important in cohesion.
Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts.
TLDR
This work reports that Scc2-Scc4 exists in a stable complex with the Cdc7-Drf1 protein kinase (DDK), which is known to bind pre-RCs and activate them for DNA replication.
Human SHPRH is a ubiquitin ligase for Mms2–Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen
TLDR
It is shown that SHPRH protein is a ubiquitin ligase indispensable for Mms2–Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen, which is consistent with the observation that this gene is mutated in a number of cancer cell lines, which raises the strong possibility thatSHPRH function is an important deterrent to mutagenesis and carcinogenesis in humans.
The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA
TLDR
The results support a model in which sister chromatid cohesion is linked to DNA replication, and a stoichiometric heteroheptameric Ctf18-RFC complex can be assembled by coexpressing the seven proteins in baculovirus-infected insect cells.
Purification and characterization of human DNA damage checkpoint Rad complexes
TLDR
Findings constitute biochemical support for models regarding the roles of checkpoint Rads as damage sensors in the DNA damage checkpoint response of human cells.
Crystal structure of the GINS complex and functional insights into its role in DNA replication
TLDR
The structural data presented here indicate that the GINS tetramer is a highly stable complex with multiple flexible surface regions.
A Mammalian Bromodomain Protein, Brd4, Interacts with Replication Factor C and Inhibits Progression to S Phase
TLDR
The present study suggests that Brd4 regulates cell cycle progression in part by interacting with replication factor C (RFC), the conserved five-subunit complex essential for DNA replication.
Studies on Human DNA Polymerase ϵ and GINS Complex and Their Role in DNA Replication
TLDR
The reconstitution of the human four-subunit Pol ϵ and characterization of its catalytic properties in comparison with Pol α and Pol δ are described and it is suggested that reduction in the level of Polδ may be less deleterious because of its collision-and-release role in lagging strand synthesis.
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