• Publications
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Distant Structural Homology Leads to the Functional Characterization of an Archaeal PIN Domain as an Exonuclease*
Genome sequencing projects have focused attention on the problem of discovering the functions of protein domains that are widely distributed throughout living species but which are, as yet, largelyExpand
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Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed rates.
The increase in enzymatic rates with temperature up to an optimum temperature (Topt) is widely attributed to classical Arrhenius behavior, with the decrease in enzymatic rates above Topt ascribed toExpand
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The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates
Mycobacterium tuberculosis has evolved a number of strategies to survive within the hostile environment of host phagocytes. Reactive nitrogen and oxygen intermediates (RNI and ROI) are among the mostExpand
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On the origin and evolution of thermophily: reconstruction of functional precambrian enzymes from ancestors of Bacillus.
Thermophily is thought to be a primitive trait, characteristic of early forms of life on Earth, that has been gradually lost over evolutionary time. The genus Bacillus provides an ideal model forExpand
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pKA values of carboxyl groups in the native and denatured states of barnase: the pKA values of the denatured state are on average 0.4 units lower than those of model compounds.
We have determined the pKA values of the 12 carboxyl residues in the native and denatured state of barnase by a combination of thermodynamic measurements on mutants of charged residues and NMRExpand
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Superantigens - powerful modifiers of the immune system.
Superantigens are powerful microbial toxins that activate the immune system by binding to class II major histocompatibility complex and T-cell receptor molecules. They cause a number of diseasesExpand
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OB-fold domains: a snapshot of the evolution of sequence, structure and function.
  • V. Arcus
  • Biology, Medicine
  • Current opinion in structural biology
  • 1 December 2002
The OB-fold is found in all three kingdoms and is well represented in both sequence and structural databases. The OB-fold is a five-stranded closed beta barrel and the majority of OB-fold proteinsExpand
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The PIN-domain toxin-antitoxin array in mycobacteria.
PIN-domains (homologues of the pilT N-terminal domain) are small protein domains of approximately 140 amino acids. They are found in a diverse range of organisms and recent evidence fromExpand
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A flexible and economical medium-throughput strategy for protein production and crystallization.
Large-scale structural genomics centres rely heavily on robotics to ensure that maximum throughput is achieved. However, the size and cost of these approaches is out of the reach of most academicExpand
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Thermodynamic theory explains the temperature optima of soil microbial processes and high Q10 values at low temperatures.
Our current understanding of the temperature response of biological processes in soil is based on the Arrhenius equation. This predicts an exponential increase in rate as temperature rises, whereasExpand
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