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A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei.
The most rapid method for the generation of conditional mutants in Trypanosoma brucei is the use of RNA interference. A single copy of the target sequence is cloned between two opposing T7 promotersExpand
Handling Uncertainty in Dynamic Models: The Pentose Phosphate Pathway in Trypanosoma brucei
Dynamic models of metabolism can be useful in identifying potential drug targets, especially in unicellular organisms. Expand
Transketolase in Trypanosoma brucei.
A single copy gene, encoding a protein highly similar to transketolase from other systems, was identified in the Trypanosoma brucei genome. The gene was expressed in E. coli and the purified proteinExpand
Clinical Profiles, Disease Outcome and Co-Morbidities among T. b. rhodesiense Sleeping Sickness Patients in Uganda
Background The acute form of Human African Trypanosomiasis (HAT, also known as Sleeping sickness) caused by Trypanosoma brucei rhodesiense has been shown to have a wide spectrum of focus specificExpand
Characterization and Developmentally Regulated Localization of the Mitochondrial Carrier Protein Homologue MCP6 from Trypanosoma brucei
ABSTRACT Proteins of the mitochondrial carrier family (MCF) are located mainly in the inner mitochondrial membrane and mediate the transport of a large range of metabolic intermediates. The genome ofExpand
Roles for the Trypanosoma brucei P2 Transporter in DB75 Uptake and Resistance
A novel trypanocide, 2,5-bis(4-amidinophenyl)furan (DB75), in its prodrug amidoxime-derivative form, 2,5-bis(4-amidinophenyl)furan-bis-O-methylamidoxime (DB289), is in trials as the first orallyExpand
The role of Trypanosoma brucei MRPA in melarsoprol susceptibility.
We previously showed that over-expression of Trypanosoma brucei MRPA, a member of the multidrug resistance protein family in T. brucei, reproducibly resulted in resistance to the anti-trypanosomalExpand
Community engagement strategies for genomic studies in Africa: a review of the literature
BackgroundCommunity engagement has been recognised as an important aspect of the ethical conduct of biomedical research, especially when research is focused on ethnically or culturally distinctExpand
A new erythrose 4-phosphate dehydrogenase coupled assay for transketolase.
The standard assay for transketolase (E.C has depended upon the use of D-xylulose 5-phosphate as the ketose donor substrate since the production of D-glyceraldehyde 3-phosphate can beExpand