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A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei.
TLDR
Cell lines in which the expression of T7 polymerase is under tetracycline control are described and it is shown that regulation of polymerase expression can modulate transcription from a constitutive T7 promoter. Expand
Handling Uncertainty in Dynamic Models: The Pentose Phosphate Pathway in Trypanosoma brucei
TLDR
A modeling strategy is presented that identifies robust predictions in the context of incomplete system characterization by exploring the mechanism underlying the essential function of one of the PPP enzymes, and validated by confirming the model predictions experimentally. Expand
Transketolase in Trypanosoma brucei.
TLDR
Subcellular localisation experiments revealed that while the protein was present in glycosomes it was found mainly within the cytosol and thus has a dual localisation, indicating a strong differential regulation in the Trypanosoma brucei life cycle. Expand
Clinical Profiles, Disease Outcome and Co-Morbidities among T. b. rhodesiense Sleeping Sickness Patients in Uganda
TLDR
A wide spectrum of sleeping sickness clinical presentation and disease outcome that was apparently not significantly influenced by concurrent infections is shown. Expand
Characterization and Developmentally Regulated Localization of the Mitochondrial Carrier Protein Homologue MCP6 from Trypanosoma brucei
TLDR
The characterization of MCP6, a novel T. brucei MCF protein, which lacks essential amino acids and sequence motifs conserved in metabolite transporters, and functional reconstitution and transport assays suggested that this protein indeed does not function as an ADP/ATP or ATP-Mg/Pi carrier. Expand
Roles for the Trypanosoma brucei P2 Transporter in DB75 Uptake and Resistance
TLDR
It is shown here that uptake of DB75 into Trypanosoma brucei also occurs principally via the P2 transporter, and that DB75 fluorescence localizes rapidly within the DNA-containing organelles of wild-type trypanosomes, whereas no fluorescence was observed in Δtbat1-null parasites or in the parasites selected for resistance to DB75. Expand
The role of Trypanosoma brucei MRPA in melarsoprol susceptibility.
TLDR
It is shown that depletion of MRPA by RNA interference resulted in moderate hypersensitivity to both melarsoprol and melarsen oxide, which is not an effect of drug metabolism since over-expression of MR PA alone conferred resistance to melarsan oxide and its principle metabolite, melarsin oxide, in vitro. Expand
Community engagement strategies for genomic studies in Africa: a review of the literature
TLDR
A review of the published literature found that there were several community engagement strategies that could support genomic studies in Africa and further research is needed to identify effective strategies to engage research participants and their communities beyond the participant recruitment stage. Expand
A new erythrose 4-phosphate dehydrogenase coupled assay for transketolase.
TLDR
The coupling of a transketolase reaction that converts D-fructose 6-phosphate to D-erythrose 4-ph phosphate is described, a reaction that reduces NAD+ to NADH and can be easily followed spectrophotometrically. Expand
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