V. V. Fedorchuk

Learn More
NAD+-dependent formate dehydrogenases (EC 1.2.1.2, FDH) of methylotrophic bacteria Pseudomonas sp. 101 (PseFDH) and Mycobacterium vaccae N10 (MycFDH) exhibit high homology. They differ in two amino acid residues only among a total of 400, i.e., Ile35 and Glu61 in MycFDH substitute for Thr35 and Lys61 as in PseFDH. However, the rate constant for MycFDH(More)
The kinetics of the thermal inactivation of recombinant wild-type formate dehydrogenase from Candida boidinii yeast was studied in the temperature range of 53-61(o)C and pH 6.0, 7.0, and 8.0. It was shown that the loss of the enzyme's activity proceeds via a monomolecular mechanism. Activation parameters ∆Н(-) and ∆S(-) were calculated based on the(More)
The effect of trifluoperazine, the inhibitor of serine/threonine protein kinases, and genistein, the inhibitor of tyrosine protein kinases, on the frequency of Agrobacterium-mediated transformation of angiosperms has been studied on the example of tobacco as a model plant. The study of the effects produced by trifluoperazine in a wide range of(More)
Alpha-amino acid ester hydrolase (EC 3.1.1.43, AEH) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. The AEH gene from Xanthomonas rubrilineans (XrAEH) was recently cloned in this laboratory. The three-dimensional structure of XrAEH was simulated using the homology modeling method for(More)
  • 1