V. S. Dedkov

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Putative open reading frames of MD-endonucleases have been identified in Enterobacteria genomes as a result of the search for amino acid sequences homologous to MD-endonuclease BisI. A highly conserved DNA primary structure of these open reading frames in different genera of Enterobacteria (Escherichia, Klebsiella and Cronobacter) has allowed researchers to(More)
A novel M.BstC8I DNA methylase was detected in cell lysate of Bacillus stearothermophilus C8 grown on Luria agar at 37°C. DNA methylation of bacteriophages λ and T7 in the 5′-G(m5C)NNGC-3′ segment blocked the activity of the BstC8I restrictase. The specificity of the M.BstC8I was analyzed on methylated λ DNA and using computer modeling and the data on the(More)
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