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BHK-21 cells were incubated in medium containing dopamine (DA) and catecholamine contents were then measured using the Falck cytochemical method. As compared with controls, significant increases were seen in the fluorescence of cells and these were proportional to the concentration and duration of exposure to DA and more marked in cells in suspension than(More)
We studied the effects of dopamine added to culture medium on survival of floating or adherent BHK-21 cells differing by organization of actin cytoskeleton. The viability of floating cells more drastically decreased with increasing dopamine concentration and duration of exposure than that of adherent cells. The cells worse adhered to the substrate and(More)
The effect of high temperatures on animal cells attracts the attention of researchers [1–3] in relation to the possibility of using hyperthermia as a method of anticancer therapy [4]. Both total and regional hyperthermias of tissues and organs are used [5]. One limitation of thermotherapia is the necessity to use temperatures higher than the physiologically(More)
The ultrastructure of a suspension or of adhered BHK-21 cells exposed to dopamine has been studied. It was found that ultrastructural modifications were observed mainly in cellular regions enriched in actin, including in intercellular desmosome-like contacts, microvilli, and under the membrane cortical layer. Desmosome-like contacts are hypertrophied with(More)
515 For more than 20 years, hyperthermia is regarded as a potential source of new methods of treatment of oncological diseases [1]. However, it was shown that the cells that survived after a short-term (0.5–1 h) exposure to hyperthermic conditions developed resistance to further effect of hyperthermia, which was retained in subsequent two or three days(More)
The effect of dopamine (DA) on the viability and morphology of cultured tumor THP-1 cells (human acute monocytic leukemia) was studied. DA in concentration of 10(-5) M had virtually no effect on the culture, while in concentration of 10(-4) M to 10(-3) M it stopped the growth and caused a sharp increase in cell death after 24 and 48 hours. Incubation with(More)
The possibility to reveal globular (G-) actin in cell cytosole by means of microscopy has been studied. The applicability of this method was in particular evaluated for diagnostics of malignant cells, whose main pathocytological feature is an anomalously high content of G-actin in cytosol. The cells of a common origin but with different states of cytosolic(More)
In previous papers a scheme of an apparatus with circulation of nutrient medium was presented. The apparatus was intended for culture of various cells, including primary cultures of hormone-secreting and hormone-sensitive cells on a porous support [i, 3]. Cells of the adenohypophysis and embryonic hepatocytes of rats were found to be capable of adhering,(More)
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