V. N. Shipilov

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Insulin-related peptides (IRPs) from ganglions of mollusk, Anodonta cygnea, were purified and characterized (IRP1-IRP13) using insulin and insulin-like growth factor-I (IGF-I) radioreceptor test systems. The IRPs were able to bind to insulin and IGF-I receptors. Dose-dependent curve slopes indicated that most IRPs bind with higher affinity to IGF-I(More)
For the first time, the adenylyl cyclase system (ACS) sensitive to biogenic amines in the mollusk Anodonta cygnea ganglions was revealed and characterized. Serotonin and isoproterenol stimulated AC activity and GTP-binding activity of heterotrimeric G-proteins. The AC-stimulating action of serotonin and isoproterenol was blocked by cyproheptadine and(More)
349 Type 1 diabetes mellitus (DM1) is a severe endo crine disease with a wide spectrum of complications in the CNS and peripheral organs leading to disability and premature death of patients. Though neurodegen erative diseases and related to them cognitive deficit are observed in many DM1 patients, physiological and biochemical processes of their(More)
216 Insulin has a regulatory effect on the metabolic and growth processes not only in peripheral tissues but also in the central nervous system (CNS). It had been shown that insulin controls the energy homeostasis in the brain tissue, affects the proliferation and differen tiation of neuronal cells, and is involved in the regula tion of learning and memory(More)
Cerebropleural ganglia from 4000 individuals of the mollusc Anodonta cygnea were submitted to procedures developed for isolation of vertebrate pancreatic insulins: homogenization and extraction, stage-like isoelectrical sedimentation, and ion-exchange chromatography. As a result of purification of the obtained preparation, using high-effective liquid(More)
The most common complication of diabetes mellitus of the type 1 (DM1) is a cognitive deficiency, which develops as a result of neurodegenerative changes in the brain. The aim of this work was to study the learning and spatial memory in rats with streptozotocin DM1 with different duration (1.5 and 6 months), as well as the activity of adenylyl cyclase(More)
We studied reactivity of insulin signal pathway elements, insulin receptor and insulin receptor substrate protein-2 (IRS2 protein), in rat brain in response to insulin insufficiency and insulin resistance during the development of experimental type 1 or type 2 diabetes mellitus. In type 1 diabetes mellitus characterized by acute insulin insufficiency,(More)
Diabetic peripheral neuropathy (DPN) is one of the commonest complications of type 1 diabetes mellitus (DM1). The aims of the present work were to study the dynamics of the development of pain-type DPN and the functional status of the hormone-sensitive adenylyl cyclase signal system (ACSS) in the skeletal muscle of rats with models of acute and mild DM1 and(More)
We analyzed the dynamics of neuropathic pain development and changes in catalase and superoxide dismutase (SOD) activities in the brain, liver, and skeletal muscles of male Wistar rats with 1-month streptozotocin-induced diabetes mellitus. A decrease in mechanical nociceptive threshold was revealed that progressed during the disease progress. Insulin(More)
One of the complication of type 1 diabetes mellitus (DM1) is cognitive deficit, developing as a result of neurodegenerative changes in the brain. The aims of the present work were to study learning and spatial memory in rats with streptozotocin DM1 of different durations (1.5 and 6 months) and to investigate the activity of the adenylate cyclase signal(More)