V. M. Mel’nyk

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A cytogenetic analysis of plants and tissue cultures of Gentiana lutea, G. punctata, and G. acaulis is performed. It is discovered that in vitro culturing leads to changes in the chromosome number in the calli of the species. The species specificity of the variability of the genomes of the cultured cells is demonstrated. It is established that the(More)
18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata, and G. lutea are studied by blot hybridization. A decrease in the number of repeats of ribosomal DNA by comparison with the plants is established in the callus tissues. Unlike other species, G. lutea exhibits intragenome heterogeneity of rRNA genes as well as qualitative changes(More)
Features of genetic structure and level of diversity of G. lutea populations from the Chornohora Ridge of the Ukrainian Carpathians were studied using RAPD- and ISSR-PCR. A high level of genetic diversity for studied populations concerning these DNA-markers is shown. The differences between populations are equal to 59–72% of the total genetic variation,(More)
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