V M Dipasquantonio

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Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and plasmid product levels were followed, and(More)
The growth dynamics of Escherichia coli with the temperature-sensitive plasmid, pOU140, were examined. Recombinant cells exhibited nearly identical kinetic behavior to host cells at low culture temperatures and low copy numbers. However, at higher temperatures, in which the copy number was significantly increased, the recombinant cells showed decreased(More)
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