V M Broomhead

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In a prospective study, sera from over 700 patients with suspected vasculitis, including over 200 patients undergoing renal biopsy, were examined for antibodies to neutrophil cytoplasmic antigen (ANCA). An indirect immunofluorescence assay on ethanol fixed human neutrophils identified two types of autoantibody: C-ANCA, which produces diffuse cytoplasmic(More)
Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). There is controversy over the value of(More)
Recent publications have suggested that fixing neutrophils in formalin is a useful adjunct to the differentiation of antinuclear antibodies from perinuclear antineutrophil cytoplasmic antibodies. In this small comparative study of 30 sera the use of an additional slide of formalin fixed neutrophils did not add any useful information and may be confusing as(More)
Amino acid analyses of mitochondrial membranes are compared with the amino acid composition of whole mitochondria (Alberti, 1964) and found to be very similar except in the cystine content. The composition of the endogenous amino acids found in freshly prepared mitochondria has been established and shown to differ considerably from the amino acid(More)
1. Saccharomyces cerevisiae was grown anaerobically in media with different concentrations of K(+) down to less than 1mm. Below 3.2mm the K(+) concentration limited the growth rate and yield. 2. Yeast extract was essential for maximum growth. The yield of cells when the medium contained 0.83mm-K(+) was only 30% of the yield with 90mm-K(+). 3. At the end of(More)
Recent publications have suggested that fixing neutrophils in formalin is a useful adjunct to the differentiation of antinuclear antibodies from perinuclear antineutrophil cytoplasmic antibodies. In this small comparative study of 30 sera the use of an additional slide of formalin fixed neutrophils did not add any useful information and may be confusing as(More)
1. Saccharomyces cerevisiae cells grown in limiting K(+) concentration have their growth inhibited by O(2) concentrations above 40%. With these conditions the cells grow very large and are unable to maintain ionic gradients when washed with water. 2. Cells grown in excess of K(+) showed the same pattern of change in cell size with change in O(2)(More)
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