V Lundblad

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Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit.(More)
  • V Lundblad
  • Current protocols in molecular biology
  • 2001
This unit presents a generalized protocol and describes the principles involved in cloning yeast genes by complementation in yeast. The protocol is presented using a hypothetical mutation of yeast,(More)
  • V Lundblad
  • Current protocols in molecular biology
  • 2001
This unit describes some of the most commonly used yeast vectors, as well as the cloned yeast genes that form the basis for these plasmids. Yeast vectors can be grouped into five general classes,(More)
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