V L Uziumov

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The methodological approaches of isolation of preparations of FMDV structural polypeptides to analyse them by the electrophoresis and electro-focussing methods are presented. The value of isoelectric points of protein coat of FMDV structural polypeptides and corresponding them values of electric potential are determined. The similarity and differences of(More)
It has been shown that the electron microscopy method can be used for characteristics of the electric properties of foot-and-mouth disease virus. The appearance of simultaneous positive and negative staining during the negative staining of virus preparations with 3-4% PTV solution shows the presence of full virions of constant poles designated as positively(More)
Removal of moisture from concentrated virus suspensions in a sequential manner using ultrafiltration, active ventilation and vacuum dehydration of concentrated powders enables us to obtain preparations with a residual content of moisture less than 1%, while the structure and properties of FMD virus are retained. Mechanism of inactivation of FMD virus in(More)
A method of negative staining of foot-and-mouth disease virus preparations permits to obtain separately positive (2% phosphotungstic acid solution, pH 3.0) and negative (2% PTA solution, pH 6.8 +/- 8.0) stainings. When a 3--4% PTA solution, pH 6.8 +/- 8.0 is used, simultaneous positive and negative staining of each virion is possible which characterizes the(More)