V L Semiotrochev

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A thermolabile extracellular entero-cytolysin (EC) from Vibrio cholerae non-O1 was purified by ammonium sulphate fractionation, DE-52 cellulose ion exchange chromatography, gel-filtration on Ultrogel AcA-44 and high performance liquid chromatography on a Mono Q. The purified EC had a molecular weight of 63 kD and an isoelectric point of 6.2. It was not(More)
Enzyme immunoassay was used in detection of V. cholerae cytolysin. Conjugate of immunoglobulins to purified cytolysin with horseradish peroxidase was used, obtained by the periodate technique. The method sensitivity is 2 ng/ml of purified cytolysin. The results of hemolytic activity measurements in supernatants of 40 V. cholerae strains and enzyme(More)
A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and chromatography on Mono Q. Cytolysin is shown to be a 60 kD and pI 6.2 protein. It is not inactivated by(More)