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Alkaline phosphatase from Escherichia coli (APEC) is not retained at 4 degrees C on a metal-free tris(carboxymethyl)ethylenediamine (TED) column, but at 15 degrees C the metalloenzyme becomes bound to the gel. Chromatography of phosphatase on metal-free TED gel indicates a decline in its enzymic activity and zinc content to about 26% and 40%, respectively.… (More)