V I Mel'gunov

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Annexins, the Ca(2+)- and phospholipid-binding proteins, are able to induce Ca(2+)-dependent aggregation of biomembranes. All the representatives of this family contain four or eight tandem repeats, 60-80 amino acids each. All these repeats include a highly conservative 17-member amino acid consensus sequence (an endonexin fold). The central domain(More)
Annexins belong to a family of Ca2+- and phospholipid-binding proteins that can mediate the aggregation of granules and vesicles in the presence of Ca2+. We have studied the effects of different divalent metal ions on annexin-mediated aggregation of liposomes using annexins isolated from rabbit liver and large unilamellar vesicles prepared from soybean(More)
Rabbit antibodies generated against annexins of human origin recognize a variety of rabbit own annexins. The antibodies under study can be divided into two groups. The first group comprises antibodies against human annexins I and IV-VI which are sufficiently specific and show only slight cross-reactivities with other proteins, whereas the second group(More)
The effect of ethanol and other low molecular weight alcohols having an anaesthetic action, on the activity of Ca-ATPase (EC 3.6.1.38) as well as on the Ca2+ uptake and efflux and the functional efficiency of Ca-pump in rabbit skeletal muscle sarcoplasmic reticulum membranes was studied. It was found that some alcohols, especially when taken at low(More)
The uptake of 8-C14-adenine in N. crassa strain Lindegren (+) was studied. The ability of N. crassa cells to uptake adenine from the medium reaches maximum at the very beginning of the logarithmic stage of growth. Adenine enters the mycelium against the concentration gradient. The uptake of adenine is maximal at 25-30 degrees C, pH 4,6-4,8, and adenine(More)
To determine the conditions under which the Ca-ATPase from rabbit skeletal sarcoplasmic reticulum can be restored to full activity, a systematic study of reactivation of lipid-depleted enzyme by various non-ionic detergents and surfactants has been carried out. All the non-ionic detergents used were able to reactivate the enzyme. The reactivation potencies(More)