V García-Framis

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Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity. The Vic-1 antibody was of the IgG1 subclass and demonstrated binding predominantly with the acrosomal regions(More)
Five monoclonal antibodies (MAb) with specific binding for tail, midpiece, equatorial region, and postacrosome of human sperm were selected. The reactivity of the MAbs was tested by three techniques: an ELISA with human sperm, an indirect immunostaining analyzed by a cell sorter, and immunofluorescence on the cell where the antigens were localized. The(More)
Fourteen mAb specific for human sperm membrane antigens were selected to investigate their inhibitory effect on fertilization. The antigens were not expressed in other human somatic tissues but were present in sperm from other species. The antibodies were purified from ascites fluid produced in mice. The zona-free hamster egg penetration assay was used for(More)
Sperm surface glycoproteins may be involved in sperm-zona pellucida recognition. Some of these coating proteins are of seminal plasma origin and their expression may change in the process of capacitation and acrosome reaction. Sperm specific monoclonal antibodies (mAb) define the presence and role of sperm membrane associated proteins. We have isolated a(More)
Anti-sperm antibodies in serum and seminal plasma were detected by means of an indirect immunobead test (IBT). Immunobeads with separate specificites for each immunoglobulin class (IBT-IgG, IBT-IgM, and IBT-IgA) were used. Semen parameters were controlled in all sperm donors and Biggers-Whitten-Whittingham (BWW) medium supplemented with human serum albumin(More)
Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma NS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome,(More)
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