V G Shore

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Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d less than 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d less than 1.063 g/ml fraction was examined by electron microscopy; it contained particles(More)
We measured the turnover and absorption of sitosterol and cholesterol, along with plasma sterol and lipoprotein concentrations, in one control and two subjects with sitosterolemia with xanthomatosis. All individuals consumed the same diet which contained approximately 500 mg/day of cholesterol and 250 mg/day of sitosterol. Sterol absorption was measured by(More)
Using plasma isotope-kinetic methods, we measured the absorption and turnover rates of cholesterol and sitosterol (24-ethylcholesterol) in two obligate heterozygotes (parents) and their homozygous daughter with sitosterolemia with xanthomatosis. Diets contained approximately 500 mg/day cholesterol and 100 mg/day sitosterol. In the homozygote, plasma(More)
Nondenaturing gradient gel analysis of high density lipoproteins (HDL, d 1.063-1.235 g/ml) isolated from Hep G2 24-hr, serum-free conditioned media shows four distinct, reproducible particle subclasses I, II, III, and IV with apparent Stokes' diameters of 13.3, 12.0, 9.5, and 7.4 nm, respectively. Fractions enriched in lipoproteins from each of these(More)
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