V. G. Artyukhov

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Oxidative status of rat cardiomyocytes during ischemia induced by occlusion of the descending branch of the coronary artery was studied by the methods of Fe-induced chemiluminescence and spectrophotometry of primary and secondary lipid peroxidation product. The concentrations of low-molecular-weight antioxidants a-tocopherol and citrate and activities of(More)
The effects of laser (632.8 nm) on functional and spectral properties of catalase at pH 6.0–7.4 were studied. Laser irradiation led to photoactivation of the enzyme at pH 7.1–7.4. Changes in the spectral properties of photomodified hemoprotein were found in the absorption spectrum of the protein component: apoenzyme displayed protective effects in relation(More)
Micronucleus frequency in buccal mucosa from the oral cavity of children as well as nucleolar structural characteristics (surface area of single nucleoli as well as their number and type) in the root meristem of seed progeny of birch (Betula pendula Roth) were studied in some districts of Voronezh City and Voronezh Region (Novovoronezh Town, Zemlyansk(More)
The flow-cytometric analysis of condition of human peripheral blood lymphocytes in dynamics of development of the apoptosis induced by exposure to UV-light (240-390 nanometers) at a dose of 1510 J/m2 was carried out. Superficial architectonics and changes of the level of a membrane potential and functional activity of succinatedehydrogenase of(More)
We studied the effect of UV light (240–390 nm) in doses of 75.5-4530 J/m2 on functional properties and level of intracellular messengers (cAMP, Ca2+, NO) in lymphocytic cells of the peripheral blood. Correcting effects of UV light in a dose of 151 J/m2 on cytotoxic activity of lymphocytes against Ehrlich ascetic carcinoma and calcium concentration in(More)
We observed intensification of free radical oxidation, decrease in activity, and changes in catalytic properties of aconitate hydratase in the liver of rats with toxic hepatitis. The total yield and maximum flash intensity of biochemiluminescence increased by 2.2 and 1.7 times, respectively. Differences were revealed in the regulation of aconitate hydratase(More)
It is established that ion exchange resins AV-17-2P, KU-2, AV-16-GS, AM 21A, IMAC-HP, PUROLITE and fiber VION KN-1 can be applied as carriers for inulinase immobilization. The analysis of IR spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through(More)
Using the MolScript version 2.1 computer program for protein molecule modeling and X-ray structure analysis data the spatial structures of several hydrolytic enzymes have been compared. These include glucoamylase from Aspergillus awamori and Saccharomycopsis fibuligera and lipases from Rhizopus japonicus. Results on homology of amino acid sequences and(More)
The decomposition pathways of hemoglobin UV absorption spectrum into the absorption spectra of the protein and non-protein components are proposed and substantiated by means of an additive model. We have established that the heme component has an absorption band with a maximum at λ(max) = 269.2 nm (ε = 97163) and the apoprotein component has an absorption(More)
The effects of doxycycline on the morphology and function of donor erythrocytes were studied by scanning electron microscopy. The antibiotic in concentrations of 7.8 × 10–5 and 7.8 × 10–6 mol/liter induced heterogeneous changes in the surface architectonics in the erythrocyte population. These data indicated stimulation of erythrocyte “aging” under the(More)