V. E. Barsky

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Here a simple, reproducible, and versatile method is described for manufacturing protein and ligand chips. The photo-induced copolymerization of acrylamide-based gel monomers with different probes (oligonucleotides, DNA, proteins, and low-molecular ligands) modified by the introduction of methacrylic groups takes place in drops on a glass or silicone(More)
Whole-mounted polytene chromosomes were isolated from nuclei by microdissection in 60% acetic acid and analyzed by electron microscopy. Elementary chromosome fibers in the interchromomeric regions and individual chromomeres can be distinguished in polytene chromosomes at low levels of polyteny (26–27 chromatids). Elementary fibers in the interbands are(More)
We have developed a three-component system for microbial identification that consists of (i) a universal syringe-operated silica minicolumn for successive DNA and RNA isolation, fractionation, fragmentation, fluorescent labeling, and removal of excess free label and short oligonucleotides; (ii) microarrays of immobilized oligonucleotide probes for 16S rRNA(More)
The majority of D. melanogaster salivary gland nuclei contains many nucleoli which vary in size and number. All nucleoli hybridize in situ with a cloned Drosophila DNA fragment containing 26 S ribosomal gene. Autoradiographic analysis of preparations after pulse H3-uridine or H3-thymidine labelling of the salivary gland indicates an intensive transcription(More)
We present a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application to diagnostics for genetic diseases. A robot has been constructed to manufacture sequencing "microchips." The microchip is an array of oligonucleotides immobilized into gel elements fixed on a glass plate. Hybridization(More)
The efficiency of sequencing by hybridization to an oligonucleotide microchip grows with an increase in the number and in the length of the oligonucleotides; however, such increases raise enormously the complexity of the microchip and decrease the accuracy of hybridization. We have been developing the technique of contiguous stacking hybridization (CSH) to(More)
The location of different cloned transposable elements was determined in the polytene chromosomes of gt w a and Oregon RC inbred Drosophila melanogaster stocks. Each stock was found to have about 300 hybridization sites for 12 different clones. The arrangement of the clones in the chromosomes was identical in individuals of the same stock but differed(More)
RNA synthesis following 3H-uridine pulse labeling has been studied autoradiographically in the 1B-3C region of D. melanogaster salivary gland X-chromosome. To improve the precision of RNA synthesis site localization in bands and interbands a micromanipulator technique of chromosome stretching was used. No significant RNA synthesis was detected in the(More)
The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic(More)