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Metaphase chromosomes of the Chinese hamster differentially-condensed under the influence of a) 5-bromdeoxyuridine, b) colcemide, and c) cold, were stained with acridine-orange (AO) in concentrations of 1.5 X 10(-7) to 3 X 10(-5) g/ml at pH 4.1 to 8.5. It was found that stretched chromosomal segments fluoresced in the orange/red part of the spectrum,… (More)
During the final 5.5th to 7th hr of the cycle in presence of BUdR, the human Y-chromosome was delayed in its mitotic condensation in the distal part of the long arm. This part was not homogeneous by the degree of the delay. The unevenly condensed Y-chromosome stained with quinacrine was brightly fluorescent in its stretched part. The fluorescence seemed to… (More)
Differences in the morphology of the human iso-X-chromosome differentially condensed by the treatment with 5-bromodeoxyuridine were revealed by means of quantitative morphometry. It is shown that these differences are not random and are correlated with the asynchrony of the reduplication of iso-arms. It is assumed that the differences in the structural… (More)
A modified method for fluorochrome staining with acridine orange of chromosome preparations for revealing the BUdR label is described. It has been shown that the alkaline medium used increases the contrast between chromosome areas with varying BUdR content and photoresistance of the staining.
The population study of nucleus organizing regions (NOR) activity in individuals of Georgian nationality was carried out using the Ag-method. The results obtained were compared with those for other populations: Russian, Viena-Ulm, Estonian etc. The mean number of active NORs for Georgians is 9.0, which significantly exceeds analogous indexes for the rest of… (More)