V. A. Andryushina

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Conditions of conversion of 17α-methyltestosterone to methandrostenolone with the presence of modified β-cyclodextrins (methylcyclodextrin, hydroxypropylcyclodextrin, and hydroxyethylcyclodextrin) in the steroid: cyclodextrin ratio 1: 1 were studied. The experimental solutions of modified β-cyclodextrins were prepared in deionized water with 5–7% methanol.(More)
Hydroxylation activity of the mold fungi belonging to the orders Dothideales, Hypocreales, and Mucorales towards Δ5-3β-hydroxysteroids was studied. The fungi Bipolaris sorokiniana, Fusarium sp., and Rhizopus nigricans were able to introduce hydroxy group at position 7α; however, this ability was detected only at a low substrate load and with a low yield. A(More)
Soybean sterols were converted into androst-4-ene-3,17-dione (AD) and 9α-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) using three actinobacterium strains. The transformation of a microcrystallic substrate (particle size 5–15 μm) or the transformation in the presence of randomly methylated β-cyclodextrin (MCD) were carried out by Mycobacterium neoaurum with a(More)
A product of microbiological cleavage of the sterols side chain, androsta-1,4-diene-3,17-dione, is toxic for bacteria, in particular, actinobacteria of the genera Mycobacterium and Arthrobacter. Sterols were transformed into androsta-1,4-diene-3,17-dione by culturing the M. neoaurum VKPM Ac-1656 strain in a high yield, provided that a sorbent was used for(More)
Optimum conditions for transformation of phytosterols by Mycobacterium neoaurum, required for selective cleavage of the lateral chain into androstenedione, were shown to differ from the known conditions of animal sterol (cholesterol) transformation. Complete conversion of phytosterols into androstenedione at a substrate load of no less than 20 g/l was(More)
9α-Hydroxy derivatives were prepared from 11 steroids of androstane and pregnane series using Rhodococcus erythropolis VKPM Ac-1740 culture with 0.5–10 g/l substrate concentration in the reaction mixture. 9α-Monohydroxylation proceeded regardless of the substituent structure at C17. However, the structure of the steroid molecule influenced the time of(More)
The hydroxylase activities of new strains such as Curvularia lunata, C. geniculata, C. eragrostidis, C. prasadii, Ulocladium botrytis, Alternaria tenuis, and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (DAA), were characterized. The 9α-hydroxylase activity of C. lunata(More)
The conditions for preparing 14α-hydroxyandrostenedione - an intermediate in the synthesis of highly active gestagens - using the fungus Curvularia lunata CBE with an increased steroid substrate load of 5 g/liter in the presence of methylcyclodextrin were identified. The microbiological method of introducing 14α-hydroxy groups into the androstane structure(More)
It has been demonstrated that the mycelium of Curvularia lunata at the end of the logarithmic growth phase displays a maximal 11β-hydroxylase activity towards cortexolone (4–6 g/l) used for transformation as a microcrystalline suspension in phosphate buffer. The mycelium at a later stage of fungal growth displays an elevated 14α-hydroxylase activity,(More)
9α-Hydroxyandrost-4-en-3,17-dione was prepared from a mixture of phytosterols using microbiological methods to degrade soybean sterols to androstenedione followed by its 9α-hydroxylation. Phytosterols could be extracted from deodorizer distillate sludge produced during production of soybean oil with low sterol content (5.2 %) with a level of extraction of(More)