Věra Čapková

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The amount of proteins soluble upon boiling (especially WCS120 proteins) and the ability to develop frost tolerance (FT) after cold acclimation was studied in two frost-tolerant winter wheat cultivars, Mironovskaya 808 and Bezostaya 1. Protein gel blot analysis, mass spectrometry (MS) and image analysis of two-dimensional gel electrophoresis (2-DE) gels(More)
Many flowering plants produce bicellular pollen. The two cells of the pollen grain are destined for separate fates in the male gametophyte, which provides a unique opportunity to study genetic interactions that govern guided single-cell polar expansion of the growing pollen tube and the coordinated control of germ cell division and sperm cell fate(More)
Tobacco (Nicotiana tabacum L.) microspores at the time of mitosis are characterized by the abundant occurrence of 92- and 98-kDa glycoproteins (GP92 and GP98). GP92 is a soluble protein while GP98 is bound to the insoluble microspore fraction. Both glycoproteins were isolated by affinity chromatography and SDS-PAGE and analysed by MS. Peptide sequences were(More)
A new method for the isolation and sub-fractionation of mitochondria from plant tissues is described. This method was used for the analysis of proteins isolated from a pure total mitochondrial fraction and of protein complexes obtained from two mitochondrial sub-fractions, membrane-bound and matrix, from in vitro cultivated tobacco pollen tubes. The method(More)
The transition between the quiescent mature and the metabolically active germinating pollen grain most probably involves changes in protein phosphorylation status, since phosphorylation has been implicated in the regulation of many cellular processes. Given that, only a minor proportion of cellular proteins are phosphorylated at any one time, and that(More)
Photoautotrophic suspension cells of Marchantia polymorpha were grown at gas phase CO2 concentrations of 0.4% and 2.0%. At the higher CO2 concentration the chloroplast shape appeared to be modified and the cells had about 70% more chloroplasts per cell. Differences in chlorophyll content per cell were much less pronounced, indicating a reduction in(More)
Growth of tobacco (Nicotiana tabacum L.) pollen tube is controlled by post-transcriptionally regulated protein synthesis. Stored mRNA was found to be present in the form of messenger ribonucleoprotein particles (mRNPs) in both maturing and germinating pollen. During pollen dehydration in the anthers content of mRNPs strongly increased during dissociation of(More)
The mRNA-protein complexes (mRNPs, Messenger ribonucleoprotein particles) are the "couriers" of the modern eukaryotes that process, store and deliver messages (transcripts) from the nucleus to the appropriate subcellular compartments and beyond. Presence of mRNPs arbitrates the posttranscriptional control of gene expression by editing the precursor RNA to(More)
As in animals, cell–cell communication plays a pivotal role in male–female recognition during plant sexual reproduction. Prelaid peptides secreted from the female reproductive tissues guide pollen tubes towards ovules for fertilization. However, the elaborate mechanisms for this dialogue have remained elusive, particularly from the male perspective. We(More)
Plant phenotype stability during ex vitro growth, one of the main requirements of plant micropropagation, was tested on tobacco. Plants cultivated in vitro in the presence of 3 % sucrose under photon flux density (PFD) of 200 μmol m−2 s−1 (3 % HL plants) showed the best growth and photosynthetic parameters in the course of 7-day acclimation. However,(More)