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Generation and maintenance of an effective repertoire of T cell antigen receptors are essential to the immune system, yet the number of distinct T cell receptors (TCRs) expressed by the estimated 10(12) T cells in the human body is not known. In this study, TCR gene amplification and sequencing showed that there are about 10(6) different beta chains in the(More)
Listeria monocytogenes is a pathogenic intracellular microorganism whose infection induces pleiotropic biological changes associated with host cell gene expression regulation. Here we define the gene expression profiles of the human promyelocytic THP1 cell line before and after L. monocytogenes infection. Gene expression was measured on a large scale via(More)
Platelet integrin ␣ IIb ␤ 3 responds to intracel-lular signals by binding fibrinogen and triggering cy-toskeletal reorganization, but the mechanisms of ␣ IIb ␤ 3 signaling remain poorly understood. To better understand this process, we established conditions to study ␣ IIb ␤ 3 signaling in primary murine megakaryo-cytes. Unlike platelets, these platelet(More)
Integrins are transmembrane receptors involved in interactions between cells and extracellular matrix proteins. Here we show that cell adhesion regulates insulin receptor substrate-1 (IRS-1) mRNA synthesis. When fibroblasts are held in suspension, lower levels of IRS-1 mRNA, but not of IRS-2 mRNA, are detected, and this effect is due to the negative(More)
In addition to the pleckstrin homology domain and the phosphotyrosine binding domain in insulin receptor substrate (IRS)-1 and IRS-2, a region between amino acids 591 and 786 in IRS-2 (IRS-2-(591–786)) binds to the insulin receptor. Based on peptide competition studies, this region interacts with the phosphorylated regulatory loop of the insulin receptor;(More)
Platelet integrin ␣ IIb ␤ 3 responds to intracel-lular signals by binding fibrinogen and triggering cy-toskeletal reorganization, but the mechanisms of ␣ IIb ␤ 3 signaling remain poorly understood. To better understand this process, we established conditions to study ␣ IIb ␤ 3 signaling in primary murine megakaryo-cytes. Unlike platelets, these platelet(More)
The mitogen-activated protein kinase (MAP kinase) is a key participant in growth factor-stimulated intracellular events such as proliferation and differentiation. We and others have previously described a cross-talk between the MAP kinase pathway and the cAMP pathway. Indeed, in several cell lines and, in particular in fibroblasts, an increase in the level(More)
A mutation substituting a valine for phenylalanine at residue 382 in the insulin receptor alpha-subunit has been found in two sisters with a genetic form of extreme insulin resistance. This receptor mutation impairs the ability of the hormone to activate autophosphorylation of solubilized receptors and phosphorylation of substrates (Accili, D., Mosthaf, L.,(More)
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