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A TaqMan(®) one-step reverse transcription real-time PCR (RT-qPCR) assay was developed for the specific detection and relative quantitation of Grapevine fanleaf virus (GFLV), the causal agent of grapevine fanleaf degeneration disease. The assay was targeted to a conservative region located in the 2A(HP) gene of the GFLV RNA2 molecule. The assay specificity(More)
Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we(More)
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