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The lichen cyanobacterial symbiont Nostoc sp. ATCC 53789 and its close relative Nostoc sp. GSV 224 are prolific producers of natural products, generating >25 derivatives of the cryptophycin class of secondary metabolites. Cryptophycin 1, the prototypic member of the class, is a potent tubulin-depolymerizing agent, and several semisynthetic derivatives are(More)
In this Application Note we show three system configurations for recovery collection with the Agilent 1100 Series purification system. One configuration uses a 12-position/13-port valve 3 in the waste line of the fraction collector, which offers up to twelve recovery locations. Another possibility is to use a third-party fraction collector, controlled by(More)
A high-resolution UHPLC analysis of xanthones present in the extracts of the pericarp of mangosteen (Garcinia mangostana L.) was carried out on an Agilent 1290 Infinity II LC. Excellent resolution and peak capacity (300 calculated at half height) were obtained within a total analysis time, including column reconditioning, of 16.5 minutes. The main xanthone(More)
In this paper, we discuss various methods for fraction collection in high-throughput chromatography. UV-triggered fractionation allows precise cutting of peaks. However, valuable fraction collector space is wasted, because many undesired compounds are collected. In mass-triggered fraction collection, the collector space is used more efficiently, because(More)
The analysis of aflatoxins in food matrices such as grain and nuts is an important task for the food industry to ensure product safety. This Application Note shows the analysis of the four major aflatoxins after sample preparation using an immunoaffinity column, post column UV derivatization, and fluorescence detection. We show that with this method the LOD(More)
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