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Detection of hatching inhibitors and hatching factor stimulants for golden potato cyst nematode, Globodera rostochiensis, in potato root leachate
In a comparison of four potato varieties, in-soil hatch of the golden potato cyst nematode (Globodera rostochiensis) was positively correlated to in vitro hatch in response to potato root leachate (PRL), suggesting the production of hatching inhibitors (HIs) by young potato plants.
Effects of the biological nematicide, DiTera , on hatching of Globodera rostochiensis and G. pallida
Tests indicated that, when mixed with PRD, DiTera® prevented eggshell permeability change, normally caused by PRD as an initial phase in the hatching process, in a majority of eggs.
Effects of the biological nematicide, DiTera®, on movement and sensory responses of second stage juveniles of Globodera rostochiensis, and stylet activity of G. rostochiensis and fourth stage
Electrophysiological tests demonstrated that exposure to 1% DiTera® prevented sensory perception of potato root diffusate, and electropharyngeograms of stylet protractor muscle activity showed that prior exposure of fourth stage juveniles of Ditylenchus dipsaci and J2 of G. rostochiensis to 1%, significantly reduced stylet thrusting when nematodes were transferred to artificial tap water or 10 mM serotonin.
An Improved Procedure for Assaying Hatching Activity of Potato Root Diffusates Towards Globodera Rostochiensis
The optimised bioassay procedure used 340 μl test volumes and replicate sizes of five cysts, and the cumulative number of juveniles hatching per cyst after 5 days was closely positively correlated with final hatch (after 31 days).