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RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many timesExpand
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New insights into peritrophic matrix synthesis, architecture, and function.
The peritrophic matrix (PM) is a chitin and glycoprotein layer that lines the invertebrate midgut. Although structurally different, it is functionally similar to the mucous secretions of theExpand
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A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix
One‐ and two‐dimensional gel electrophoresis coupled with liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insectExpand
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Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins.
To infect per os, baculovirus virions cross the peritrophic matrix (PM) to reach the midgut epithelium. Insect intestinal mucins (IIMs) are PM proteins that protect the PM and aid passage of the foodExpand
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Characterization of the Mamestra configurata (Lepidoptera: Noctuidae) larval midgut protease complement and adaptation to feeding on artificial diet, Brassica species, and protease inhibitor.
The midgut protease profiles from 5th instar Mamestra configurata larvae fed various diets (standard artificial diet, low protein diet, low protein diet with soybean trypsin inhibitor [SBTI], orExpand
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In vitro and in vivo application of RNA interference for targeting genes involved in peritrophic matrix synthesis in a lepidopteran system
Abstract  The midgut of most insects is lined with a semipermeable acellular tube, the peritrophic matrix (PM), composed of chitin and proteins. Although various genes encoding PM proteins have beenExpand
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Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae
Insect intestinal mucins (McIIM2‐4) expressed in the midgut of feeding, starved and moulting Mamestra configurata larvae were identified. McIIM2 and McIIM4 were associated with the peritrophic matrixExpand
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A journey into the world of insect lipid metabolism.
Lipid metabolism is fundamental to life. In insects, it is critical, during reproduction, flight, starvation, and diapause. The coordination center for insect lipid metabolism is the fat body, whichExpand
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First record of a NPV isolated from Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) in Turkey and its molecular identification according to the partial lef-8 gene.
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is an important pest in Turkey and causes extensive economic losses in many cultivated crops. The viruses within the family Baculoviridae areExpand
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The Role of Peptide Hormones in Insect Lipid Metabolism
  • U. Toprak
  • Biology, Medicine
  • Frontiers in Physiology
  • 7 May 2020
Lipids are the primary storage molecules and an essential source of energy in insects during reproduction, prolonged periods of flight, starvation, and diapause. The coordination center for insectExpand
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