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Conserved and acquired features of adult neurogenesis in the zebrafish telencephalon.
Using BrdU tracing and immunodetection of cell-type-specific markers, it is demonstrated that the adult zebrafish telencephalon contains self-renewing progenitors, which show features of adult mammalian neural stem cells but distribute along the entire dorso-ventral extent of theTelencephalic ventricular zone.
Sonic hedgehog is not required for the induction of medial floor plate cells in the zebrafish.
It is concluded that shh is neither required nor sufficient to induce this cell type in the zebrafish, and ectopic overexpression of shh in zebra fish embryos does not induce ectopic medial floor plate cells.
Neurogenesis in zebrafish – from embryo to adult
The contributions of zebrafish in neural development and adult neurogenesis are summarized.
The activity of neurogenin1 is controlled by local cues in the zebrafish embryo.
Zebrafish neurogenin1 encodes a basic helix-loop-helix protein which shares structural and functional characteristics with proneural genes of Drosophila melanogaster and its expression is modulated by Delta/Notch signalling, suggesting that it is a target of lateral inhibition.
Dynamic microtubules and specification of the zebrafish embryonic axis
To form an organizer, zebrafish blastomeres appear to require substances which are transported from the vegetal hemisphere of the yolk cell by cortical microtubules, as shown by the movement of polystyrene beads.
Axial, a zebrafish gene expressed along the developing body axis, shows altered expression in cyclops mutant embryos.
Cl cloning of a cDNA from zebrafish encoding a member of the fork head/HNF3 gene family is reported, suggesting that Axial plays a crucial role in specification of both the axial mesendoderm and the ventral central nervous system.
Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo
The neuromuscular phenotype in ache mutants is suppressed by a homozygous loss-of-function allele of the α-subunit of the nicotinic acetylcholine receptor (nAChR), indicating that the impairment of neuromumuscular development is mediated by activation of nA ChR in the mutant.
Wnt5 is required for tail formation in the zebrafish embryo.
It is shown that the zebrafish pipetail gene is identical to Wnt5, which suggests a major role for wnt5 in morphogenetic processes underlying tail outgrowth and cartilage differentiation in the head.
A crucial component of the endoderm formation pathway, CASANOVA, is encoded by a novel sox-related gene.
An HMG box-containing gene involved in the fate decision between endoderm and mesoderm that acts downstream of Nodal signals is identified and identified a mutation within 10J3 coding sequence that cosegregates with the cas phenotype, clearly demonstrating that cas is encoded by 10 J3.
Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene
Two glucocorticoid response elements (GREs) located 2.5 kb upstream of the transcription initiation site of the tyrosine aminotransferase gene were identified by gene transfer experiments and shown