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Structural basis of hepatocyte growth factor/scatter factor and MET signalling.
The study shows how the proteolytic mechanism of activation of the complex proteinases has been adapted to cell signaling in vertebrate organisms, offers a description of monomeric and dimeric ligand-receptor complexes, and provides a foundation to the structural basis of HGF/SF-MET signaling. Expand
Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography.
Using electron tomography, it is shown that the slit diaphragm comprises a network of winding molecular strands with pores the same size as or smaller than albumin molecules, as demonstrated in humans, rats, and mice, and the results suggest the direct involvement of nephrin molecules in constituting the macromolecule-retaining slit diphragm and its pores. Expand
Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography
A specific premessenger ribonucleoprotein (RNP) particle in the salivary glands of the dipteran Chironomus tentans was studied with electron microscope tomography during translocation from the cellExpand
The human skin barrier is organized as stacked bilayers of fully extended ceramides with cholesterol molecules associated with the ceramide sphingoid moiety.
The determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, is reported on, using a methodological approach combining very high magnification cryo-electron microscopy of vitreous skin section defocus series, molecular modeling, and EM simulation. Expand
Nephrin promotes cell-cell adhesion through homophilic interactions.
It is shown by using a surface plasmon resonance biosensor that soluble recombinant variants of nephrin, containing the extracellular part of the protein, interact with each other in a specific and concentration-dependent manner. Expand
Structure and flexibility of individual immunoglobulin G molecules in solution.
Cryo-electron tomography is used to analyze a monoclonal IgG, with a molecular weight of only 150 kDa, and reveals y-shaped IgG molecules with three protruding subunits, most likely of functional significance in antigen recognition. Expand
In situ transcription and splicing in the Balbiani ring 3 gene
The structural data show that the spliceosome is not a structurally well‐defined unit in situ and that the C‐terminal domain of the elongating RNA polymerase II cannot carry splicedosomal components for all introns in the BR3 transcript. Expand
Electron Tomography Reveals Posttranscriptional Binding of Pre-Mrnps to Specific Fibers in the Nucleoplasm
Observations indicate that pre-mRNPs are not always freely diffusible in the nucleoplasm but interact with fibers of specific structure and composition, which implies that some of the posttranscriptional events that the pre- mRNPs undergo before reaching the NPC occur in a bound state. Expand
The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters
Structural analyses reveal that oligomerization between cell adhesion molecules in the same membrane is influenced by their interactions across opposing membranes (see also in this issue theExpand
Structural interaction between the nuclear pore complex and a specific translocating RNP particle
The transport of Balbiani ring (BR) premessenger RNP particles in the larval salivary gland cells of the dipteran Chironomus tentans can be followed using electron microscopy and it was concluded that the particle-nuclear ring interactions reflect a rotation of the ribbon ring in front of the central channel. Expand