Structural basis of hepatocyte growth factor/scatter factor and MET signalling.
- E. Gherardi, S. Sandin, D. Svergun
- BiologyProceedings of the National Academy of Sciences…
- 14 March 2006
The study shows how the proteolytic mechanism of activation of the complex proteinases has been adapted to cell signaling in vertebrate organisms, offers a description of monomeric and dimeric ligand-receptor complexes, and provides a foundation to the structural basis of HGF/SF-MET signaling.
The human skin barrier is organized as stacked bilayers of fully extended ceramides with cholesterol molecules associated with the ceramide sphingoid moiety.
- I. Iwai, HongMei Han, L. Norlén
- Biology, ChemistryJournal of Investigative Dermatology
- 1 September 2012
The determination of the molecular organization of the skin's lipid matrix in situ, in its near-native state, is reported on, using a methodological approach combining very high magnification cryo-electron microscopy of vitreous skin section defocus series, molecular modeling, and EM simulation.
Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography.
- J. Wartiovaara, L. Ofverstedt, K. Tryggvason
- Biology, MedicineJournal of Clinical Investigation
- 15 November 2004
Using electron tomography, it is shown that the slit diaphragm comprises a network of winding molecular strands with pores the same size as or smaller than albumin molecules, as demonstrated in humans, rats, and mice, and the results suggest the direct involvement of nephrin molecules in constituting the macromolecule-retaining slit diphragm and its pores.
Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography
Nephrin promotes cell-cell adhesion through homophilic interactions.
In situ transcription and splicing in the Balbiani ring 3 gene
The structural data show that the spliceosome is not a structurally well‐defined unit in situ and that the C‐terminal domain of the elongating RNA polymerase II cannot carry splicedosomal components for all introns in the BR3 transcript.
Electron Tomography Reveals Posttranscriptional Binding of Pre-Mrnps to Specific Fibers in the Nucleoplasm
Observations indicate that pre-mRNPs are not always freely diffusible in the nucleoplasm but interact with fibers of specific structure and composition, which implies that some of the posttranscriptional events that the pre- mRNPs undergo before reaching the NPC occur in a bound state.
The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters
Structural analyses reveal that oligomerization between cell adhesion molecules in the same membrane is influenced by their interactions across opposing membranes (see also in this issue the…
Structure and flexibility of individual immunoglobulin G molecules in solution.
Spatial visualization of the maturing HIV-1 core and its linkage to the envelope.
The core of late states of maturing human immunodeficiency virus type 1 (HIV-1) has been visualized in three dimensions at approximately 7 nm resolution by electron microscopic tomography. After…