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Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: a validated assay
Cytochrome P450 BM-3 (CYP102) catalyzes the subterminal hydroxylation of fatty acids with a chain length of 12–22 carbons. The paper focuses on the regioselectivity and substrate specificity of theExpand
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Stereoselective epoxidation of the last double bond of polyunsaturated fatty acids by human cytochromes P450
Cytochromes P450 (CYPs) metabolize polyunsaturated long-chain fatty acids (PUFA-LC) to several classes of oxygenated metabolites. Through use of human recombinant CYPs, we recently showed thatExpand
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Protein Engineering – An Option for Enzymatic Biofuel Cell Design
This review summarizes and discusses from a protein engineering point of view strategies to improve performances of biocatalysts in enzymatic biofuel cells. Expand
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Directed laccase evolution for improved ionic liquid resistance
In nature, the biodegradation of lignin is a challenging process since lignin is highly cross-linked and poorly water-soluble. Laccases (EC, benzenediol: oxygen oxidoreductase) play a keyExpand
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Grafting PNIPAAm from β-barrel shaped transmembrane nanopores.
The research on protein-polymer conjugates by grafting from the surface of proteins has gained significant interest in the last decade. While there are many studies with globular proteins, membraneExpand
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Structural Insight into Enantioselective Inversion of an Alcohol Dehydrogenase Reveals a "Polar Gate" in Stereorecognition of Diaryl Ketones.
Diaryl ketones are important building blocks for synthesizing pharmaceuticals and are generally regarded as “difficult-to-reduce” ketones due to the large steric hindrance of their two bulky aromaticExpand
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Ionic liquid effects on the activity of monooxygenase P450 BM-3
P450 monooxygenases oxidize a broad range of substrates including fatty acids, alcohols, aliphatic and aromatic hydrocarbons which are often poorly soluble in water and have Km values in theExpand
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In Vitro Double Oxidation of n-Heptane with Direct Cofactor Regeneration
A novel concept for the direct oxidation of cycloalkanes to the corresponding cyclic ketones in a one-pot synthesis in water with molecular oxygen as sole oxidizing agent was reported recently. BasedExpand
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A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein
Whole cell catalysis is, in many cases, a prerequisite for the cost-effective production of chemicals by biotechnological means. Synthetic metal catalysts for bioorthogonal reactions can beExpand
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Prearranged glycosides, 2. Intramolecular glycosylation of prearranged saccharides as a novel strategy for the construction of β‐L‐rhamnosidic linkages
Disaccharides containing a β-L-rhamnosyl residue were prepared by a novel intramolecular glycosylation strategy. Thus, suitably benzyl-protected L-rhamnosyl donors (ethyl and phenyl 1-thiorhamnosideExpand
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