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Crystal structure of the 14-subunit RNA polymerase I
The crystal structure of Pol I from Saccharomyces cerevisiae at 3.0 Å resolution shows a compact core with a wide DNA-binding cleft and a tightly anchored stalk, and an extended loop mimics the DNA backbone in the clefts and may be involved in regulating Pol I transcription.
Conformational flexibility of RNA polymerase III during transcriptional elongation
The transcribing Pol III enzyme structure not only shows the complete incoming DNA duplex, but also reveals the exit path of newly synthesized RNA, which likely increases processivity and provides structural insights into the conformational switch between Pol III‐mediated initiation and elongation.
Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry
The study reveals ABCE1 bound to the translational factor-binding (GTPase) site with multiple cross-link contacts of the helix–loop–helix motif to the S24e ribosomal protein.
Analyzing RNA polymerase III by electron cryomicroscopy
The structure of the free Pol III enzyme at 10 Å resolution provides an accurate framework to better understand its overall architecture and the structural organization and functional role of two Pol III-specific subcomplexes.
Protein minimization of the gp120 binding region of human CD4.
A mutational strategy was designed to express CD4D1 and region 21-64 of CD4 (CD4PEP1) in Escherichia coli and these CD4 derivatives should be useful tools in HIV vaccine design and entry inhibition studies.
Automated structure modeling of large protein assemblies using crosslinks as distance restraints
An automated modeling method dedicated to large protein assemblies that uses a form of spatial restraints that realistically reflects the distribution of experimentally observed crosslinked distances, and automatically deals with ambiguous and/or conflicting crosslinks and identifies alternative conformations within a Bayesian framework is introduced.
Characterization of gp120 and Its Single-Chain Derivatives, gp120-CD4D12 and gp120-M9: Implications for Targeting the CD4i Epitope in Human Immunodeficiency Virus Vaccine Design
Although antibodies targeting the CD4i epitope were generated by the gp120-CD4D12 immunogen, these antibodies were nonneutralizing, suggesting that the broadly neutralizing response observed is exclusively due to anti- CD4 antibodies.
Structure of Aquifex aeolicus Argonaute Highlights Conformational Flexibility of the PAZ Domain as a Potential Regulator of RNA-induced Silencing Complex Function*
- U. Rashid, Dirk Paterok, A. Koglin, H. Gohlke, J. Piehler, J. Chen
- ChemistryJournal of Biological Chemistry
- 4 May 2007
Conformational rearrangement of the PAZ domain may be critical for the catalytic cycle of Argonaute and the RNA-induced silencing complex.
Multiple targets for suppression of RNA interference by tomato aspermy virus protein 2B.
The nucleic acid binding properties of the Cucumovirus RNAi suppressor tomato aspermy virus protein 2B (TAV 2B) are reported, showing that TAV 2B binds double-stranded RNA corresponding to siRNAs and miRNAs, as well as single-Stranded RNA oligonucleotides.