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PCR primers to amplify 16S rRNA genes from cyanobacteria
The use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities is demonstrated. Expand
Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis
The results demonstrate the usefulness of TGGE for the structural analysis of heterogeneous rRNA genes together with their expression, stress problems of the generation of meaningful data for 16S rRNA sequences and probe designs, and might have consequences for evolutionary concepts. Expand
Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus
The evolutionary history of a major new animal pathogen that has undergone rapid avian host adaptation and intercontinental dissemination is determined, providing a new paradigm for the impact of human activities on the emergence of animal pathogens. Expand
Phylogenetic and Morphological Diversity of Cyanobacteria in Soil Desert Crusts from the Colorado Plateau
The diversity found in desert crusts was underrepresented in currently available nucleotide sequence databases, and several novel phylogenetic clusters could be identified, indicating that soil characteristics may select for specific cyanobacteria. Expand
Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria
There was excellent correlation between the broth microdilution assay and detection of antibiotic resistance genes by the multiplex PCR, in the determination of S. aureus resistance to erythromycin, gentamicin, methicillin and tetracycline. Expand
The phylogeny of unicellular, extremely halotolerant cyanobacteria
This represents the first case in which a phylogenetically coherent group of cyanobacteria can be defined on the basis of physiology, and the Halothece cluster contained two subclusters that may be divergent at the generic level. Expand
Whole Genome Sequencing versus Traditional Genotyping for Investigation of a Mycobacterium tuberculosis Outbreak: A Longitudinal Molecular Epidemiological Study
In an outbreak investigation of Mycobacterium tuberculosis comparing whole genome sequencing (WGS) with traditional genotyping, Stefan Niemann and colleagues found that classical genotyping falselyExpand
A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic.
The genetic changes associated with adaptation to the hospital environment and with increasing drug resistance over time are document, and how MRSA evolution likely has been influenced by country-specific drug use regimens are documented. Expand
Emergence of methicillin-resistant Staphylococcus aureus (MRSA) in different animal species.
The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in animals such as horses, pet animals and productive livestock has raised questions of a probable human origin and in more generalExpand