• Publications
  • Influence
Nonhomologous end joining and homologous recombination DNA repair pathways in integration mutagenesis in the xylose-fermenting yeast Pichia stipitis.
The results suggest that in P. stipitis the nonhomologous end joining (NHEJ) pathway obviously predominates the homologous recombination pathway of double-strand break repair. Expand
Distribution of alkB genes within n‐alkane‐degrading bacteria
Fifty‐four bacterial strains belonging to 37 species were tested for their ability to assimilate short chain and/or medium chain liquid n‐alkanes and the derived amino acid sequence of the alkB‐amplificate of Pseudomonas aureofaciens showed high homology with AlkB from Ps. Expand
Influence of carbon and nitrogen concentration on itaconic acid production by the smut fungus Ustilago maydis
Itaconic acid is a valuable platform compound for the production of bio‐based polymers, chemicals, and fuels. Ustilago maydis is a promising host for the production of itaconic acid fromExpand
Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia stipitis and identification of the fermentative ADH
Two Pichia stipitis ADH genes (PsADH1 and PsADH2) were isolated by complementation of a Saccharomyces cerevisiae Adh−‐mutant. The genes enabled the transformants to grow in the presence of antimycinExpand
Genetic aspects of targeted insertion mutagenesis in yeasts.
The behaviour of "non-conventional" yeast species, which show specific properties of special interest to both basic and applied research, are reviewed and similar approaches based on homologous recombination that have been established for the mitochondrial genome of S. cerevisiae are described. Expand
Competition between n-alkane-assimilating yeasts and bacteria during colonization of sandy soil microcosms
It is confirmed that yeasts overgrow bacteria in sandy soil, one of the colonization factors useful for the yeasts, and can be counteracted by addition of bentonite, a clay mineral with high ion exchange capacity, but not, however, by kaolin. Expand
Peculiarities of the regulation of fermentation and respiration in the crabtree-negative, xylose-fermenting yeast Pichia stipitis.
The respiration of Pichia stipitis was not repressed by either high concentrations of fermentable sugars or oxygen limitation, and pyruvate decarboxylase was not inhibited by phosphate. Expand
Characterization of the genetic system of the xylose-fermenting yeast Pichia stipitis
High mutant frequencies indicated that the wild-type strains of Pichia stipitis are haploid, andSporulation ability of these clones pointed to a homothallic life cycle, although asci contained preferentially spore dyads. Expand
A mutation in the COX5 gene of the yeast Scheffersomyces stipitis alters utilization of amino acids as carbon source, ethanol formation and activity of cyanide insensitive respiration
Scheffersomyces stipitis PJH was mutagenized by random integrative mutagenesis and the integrants were screened for lacking the ability to grow with glutamate as sole carbon source. One of the twoExpand