U. K. Laemmli

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

U. K. Laemmli
Nature
1970

Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major… (More)

Maturation of the head of bacteriophage T4. I. DNA packaging events.

U. K. Laemmli, M. Favre
Journal of molecular biology
1973

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Don W Cleveland, S. G. Fischer, Marc W Kirschner, U. K. Laemmli
The Journal of biological chemistry
1977

A rapid and convenient method for peptide mapping of proteins has been developed. The technique, which is especially suitable for analysis of proteins that have been isolated from gels containg… (More)

The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.

Hirohito Haruki, Junichi Nishikawa, U. K. Laemmli
Molecular cell
2008

The anchor-away (AA) technique depletes the nucleus of Saccharomyces cerevisiae of a protein of interest (the target) by conditional tethering to an abundant cytoplasmic protein (the anchor) by… (More)

Nup-PI: the nucleopore-promoter interaction of genes in yeast.

Manfred Schmid, Ghislaine Arib, Caroline M. Laemmli, Junichi Nishikawa, Thérèse Durussel, U. K. Laemmli
Molecular cell
2006

Our previous work identified the inner basket of the NPC as a physical activation/protection station for force-tethered, epigenetically silenced genes. Here we show that a specific… (More)

Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.

Jovan Mirkovitch, M. E. Mirault, U. K. Laemmli
Cell
1984

Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells. We find one loop for each of the tandemly repeated histone gene… (More)

Higher order metaphase chromosome structure: evidence for metalloprotein interactions.

One level of DNA organization in metaphase chromosomes is brought about by a scaffolding structure that is stabilized by metalloprotein interactions. Fast-sedimenting, histone-depleted structures… (More)

Chromatin Boundaries in Budding Yeast The Nuclear Pore Connection

Kojiro Ishii, Ghislaine Arib, Clayton Lin, Griet van Houwe, U. K. Laemmli
Cell
2002

Chromatin boundary activities (BAs) were identified in Saccharomyces cerevisiae by genetic screening. Such BAs bound to sites flanking a reporter gene establish a nonsilenced domain within the silent… (More)

Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.

We find DNA fragments attached to the nuclear scaffold (SARs) both 5' and 3' of three Drosophila genes, defining looped domains ranging from 4.5 to 13 kb. For the two-promoter-containing gene Adh… (More)

A factor preventing the major head protein of bacteriophage T4 from random aggregation.

U. K. Laemmli, F X Béguin, G Gujer-Kellenberger
Journal of molecular biology
1970

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