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Annealing of RNA editing substrates facilitated by guide RNA‐binding protein gBP21
Using recombinant gBP21 preparations, it is shown that the protein stimulates the annealing of gRNAs to cognate pre‐mRNAs in vitro, andKinetic data establish an enhancement of the second order rate constant for the gRNA–pre‐mRNA interaction.
The involvement of gRNA-binding protein gBP21 in RNA editing-an in vitro and in vivo analysis.
It is established that gBP21 contributes a non-essential function to the RNA editing reaction and further suggest that the protein is involved in additional mitochondrial processes which impact a larger pool of mitochondrial transcripts.
Mechanism of the gBP21-mediated RNA/RNA annealing reaction: matchmaking and charge reduction.
It is concluded that gBP21 works as a matchmaker by binding to gRNAs as one of the two RNA annealing reactants, and three lines of evidence substantiate this model.
Re-creating an RNA world
- U. F. Müller
- BiologyCellular and Molecular Life Sciences CMLS
- 2 May 2006
This review summarizes the recent progress made in the development of an RNA world and outlines the obstacles that remain to be overcome.
TbMP42, a protein component of the RNA editing complex in African trypanosomes, has endo-exoribonuclease activity.
Improved polymerase ribozyme efficiency on hydrophobic assemblies.
It is shown here that the hydrophobic anchors led to aggregates with the expected size of the corresponding micelles, which increased the polymerization yield of full-length products by 3- to 20-fold, depending on substrates and reaction conditions.
An in vivo selection method to optimize trans-splicing ribozymes.
This method uses an in vivo rescue assay where the mRNA of an inactivated antibiotic resistance gene is repaired by trans-splicing group I intron ribozymes, and this in vivo selection method can now be used to optimize any sequence in trans- Splicing riboz enzymes.
Mapping a Systematic Ribozyme Fitness Landscape Reveals a Frustrated Evolutionary Network for Self-Aminoacylating RNA
The method (SCAPE: sequencing to measure catalytic activity paired with in vitro evolution) shows that the landscape contains three major ribozyme families (landscape peaks), and the frustrated nature of the evolutionary network suggests that chance emergence of a ribo enzyme motif would be more important than optimization by natural selection.
Substrate 2'-hydroxyl groups required for ribozyme-catalyzed polymerization.
Spliceozymes: Ribozymes that Remove Introns from Pre-mRNAs in Trans
A new variant of the group I intron ribozyme from Tetrahymena is described that recognizes two splice sites on a substrate RNA, removes the intron sequences between thesplice sites, and joins the flanking exons, analogous to the action of the spliceosome.