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Superresolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes
Significance The structure of the pachytene chromosome is essential to understand how genetic recombination can happen during meiosis. Using single molecule localization microscopy with DNA staining,Expand
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Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes
Several approaches have been described to fluorescently label and image DNA and chromatin in situ on the single-molecule level. These superresolution microscopy techniques are based on detectingExpand
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Nanostructure analysis using spatially modulated illumination microscopy
We describe the usage of the spatially modulated illumination (SMI) microscope to estimate the sizes (and/or positions) of fluorescently labeled cellular nanostructures, including a briefExpand
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Combination of structured illumination and single molecule localization microscopy in one setup
TLDR
We present a high-resolution fluorescence microscope setup which combines the advantages of SMLM with SIM in order to provide high-precision localization and structural information in a single setup. Expand
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A transient ischemic environment induces reversible compaction of chromatin
BackgroundCells detect and adapt to hypoxic and nutritional stress through immediate transcriptional, translational and metabolic responses. The environmental effects of ischemia on chromatinExpand
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Localization microscopy of DNA in situ using Vybrant(®) DyeCycle™ Violet fluorescent probe: A new approach to study nuclear nanostructure at single molecule resolution.
Higher order chromatin structure is not only required to compact and spatially arrange long chromatids within a nucleus, but have also important functional roles, including control of gene expressionExpand
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Super-resolution binding activated localization microscopy through reversible change of DNA conformation
ABSTRACT Methods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments haveExpand
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Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe
Single Molecule Localization Microscopy (SMLM) is a recently emerged optical imaging method that was shown to achieve a resolution in the order of tens of nanometers in intact cells. Novel highExpand
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Spatially modulated illumination microscopy using one objective lens
The spatially modulated illumination (SMI) microscope is a wide-field fluorescence microscope featuring axially structured illumination, through which access to information about subresolution objectExpand
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Super-resolution microscopy approaches to nuclear nanostructure imaging.
The human genome has been decoded, but we are still far from understanding the regulation of all gene activities. A largely unexplained role in these regulatory mechanisms is played by the spatialExpand
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