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Antisera were raised in rabbits using highly purified urotensin I (UI) or UI (4-41) conjugated with high-molecular-weight proteins. Antiserum 2U4, at a final dilution of 1:300,000, gave 50% binding to iodinated UI (specific activity 40-120 microCi/micrograms). Carp (Cyprinus carpio) and sucker (Catostomus commersoni) UI peptides cross-reacted completely(More)
Glia cells of rat neural lobes (pituicytes) were stained in thin sections (6 micrometers) by the indirect immunofluorescence technique for the glial fibrillary acidic protein (GFA), the S-100 protein and fibronectin. The positive strong GFA-staining of the pituicytes demonstrates their astroglial character. Fibronectin was located along the blood(More)
Bovine oocytes aspirated from small follicles were cultured for various time periods. Subsequently, the oocytes were fixed and stained with Giemsa and analyzed for their chromosome configuration. The appearance of the various chromosome configurations and their time sequence were documented. The influence of the presence or absence of follicle-stimulating(More)
Radioimmunoassay and chemical assay (high performance liquid chromatography (HPLC) with fluorescence detection) for arginine vasopressin (AVP) in purified synthetic preparations and in posterior lobe tissue samples (ox and rat) yielded essentially identical values. In tissue samples from the neurohypophysis, the reproducibility of the former method was(More)
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