Tzvee N. Harris

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Because staphylococcal protein A binds all the known subclasses of mouse IgG except IgG1, ethanol-fixed staphylococci were used as an adsorbent to prepare IgG1 fractions of anti-BALB/c alloantibody-containing globulins and normal globulins of the same strains. The loss of more than 99% of the IgG2 as a result of this adsorption was demonstrated by(More)
Antibody-producing cells have been identified among cells obtained from efferent lymphatic vessels, the thoracic duct, and peripheral blood. These cells, which produced plaques of hemolysis and which were quite rare (20 to 50 per million), due in most instances to 19S antibody, were located and studied by electron microscopy. Of the antibody-producing cells(More)
Lymph node cells of rabbits injected with sheep erythrocytes, identified as antibody-producing by their ability to produce plaques of hemolysis in erythrocyte-containing agar layers, have been examined by electron microscopy, by the use of a procedure devised for subjecting single cells to such examination. The antibody-producing cells thus examined were(More)
It was demonstrated by Keogh et al. (1947) and Warburton et al. (1949) that several products of bacterial cultures could be adsorbed to erythrocytes, which then could be agglutinated by sera containing antibodies to those bacterial antigens. This approach was developed in the case of a constituent of tuberculin by Middlebrook and Dubos (1948) and Rothbard(More)
In studies of the mechanism of lysis of red blood cells by washed streptococci with hemolytic activity (cell-bound hemolysin, CBH) no components released spontaneously by RBC or streptococci, or by interaction between these cells, could be found to induce the formation of soluble hemolysin by the streptococci. It was also found that separation of RBC from(More)
I t was shown that although a variety of unrelated materials such as RNA, serum albumin, serum a lipoprotein, tween 40, and triton X-205 could induce the production of hemolysin from resting streptococci, a number of similarities could be demonstrated among the resulting preparations of hemolysin. All were inhibited by lecithin, trypan blue, congo red,(More)