Tuyoshi Ozaki

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Endothelins modulate hormonal secretion in the pituitary gland. Intense signaling of endothelin A receptors (ETAR) has been detected by in situ hybridization, binding assay and receptor autoradiography. We used light- and electron-microscopic immunohistochemistry of ETAR with polyclonal antibody against a synthetic peptide corresponding to the carboxyl(More)
Intense expression of mRNA of endothelin-B receptor (ETBR) has been detected in the Bergmann glia of cerebellum by in situ hybridization, but the intracellular localization has not been reported because of the absence of a useful antibody for immunohistochemical investigations. We made polyclonal antibodies against the carboxyl terminus of human ETBR(More)
The immunoreaction of a monoclonal antibody (Mab) and an isolated synaptic vesicle (SV) was processed on a grid mesh and the result could be easily observed with electron microscopy. The SV suspension was obtained and dispersed on the grid mesh where immunoreaction procedures were performed. The resulting immunoreaction was visualized by labelling with(More)
The cytotoxicity and biological safety of calcium phosphate glass ceramics (CPGC) blocks and powders were examined. CPGC blocks were implanted in the femur, muscle and subcutaneous tissue, and CPGC powders in the medullary cavity, joint, subcutaneous tissue and the abdominal cavity of rats. These rats were sacrificed one or two years after implantation,(More)
Spheroid and flattened synaptic vesicles were isolated from the brain homogenate of guinea pigs by a modified purification method. For scanning and transmission electron microscopy, a simple dipping method of preparation was developed and used. The purest and richest fraction of synaptic vesicles was obtained from a 0.1 M sucrose fraction of density(More)
We report on the microscopic surface structure of carbon-fiber-reinforced silicon carbide (C/SiC) composite mirrors that have been improved for the Space Infrared Telescope for Cosmology and Astrophysics (SPICA) and other cooled telescopes. The C/SiC composite consists of carbon fiber, silicon carbide, and residual silicon. Specific microscopic structures(More)
In this paper, we describe the influence of monocarboxylic acids (formic acid and acetic acid) and dicarboxylic acids (succinic acid and adipic acid), which are usually contained in aerosol particles and fog water, on the growth of tobacco plant. Their influence was examined by spraying the acid solutions on intact plants and by administering them in a(More)
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