Tuija Lahtinen

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It has been demonstrated both in vivo and in vitro that cytomegalovirus regulates not only MHC class I but also class II antigen expression on vascular endothelial cells. The CMV-linked MHC induction is believed to be involved in acute rejection mechanisms and chronic vasculopathy after transplantation. In this study, we have investigated the effect of(More)
Explants of anterior, intermediate and posterior pituitary lobes of rats at various ages evoked an intense nerve fibre outgrowth when co-cultured with neonatal sympathetic superior cervical ganglia in a collagen gel medium. Freezing and thawing of pituitary lobes, prior to culture, did not abolish their growth-promoting effect. Since the addition of(More)
Nerve growth factor (NGF)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-NGF isolated from adult male mouse submaxillary salivary gland. Co-culture of frozen or fresh intermediate lobes with newborn rat superior cervical ganglion resulted in marked fiber(More)
5-Hydroxytryptamine was demonstrated immunohistochemically in developing sympathetic chain ganglia, adrenal medullae and retroperitoneal paraganglionic tissue. Initially, all catecholamine-synthesizing cells were immunoreactive to 5-hydroxytryptamine, but during the maturation some sympathetic cell types lose this property. In the ganglia, small intensely(More)
AIMS To measure physicians' use of the alcometer (breath analysis test) and their documentation of alcohol-related findings among their patients. METHODS Over one weekend, during which 100 adults attended the emergency clinic of a university hospital, data were collected on patients' alcohol consumption, physicians' use of an alcometer and alcohol-related(More)
Sympathetic chain ganglia of 3-day-old rats were cultured in Rose chambers for 5 days. Each chamber contained either two ganglia or a ganglion and a piece of heart atrium. Visual examination failed to show an increased density or length of nerve fibers growing towards the other explant. However, actual measurements showed that the length of nerve fiber(More)
Whole sympathetic superior cervical ganglia of 14- and 15-day-old rat embryos were cultured in a collagen gel medium for 24 h with or without explants of heart atrium from the same animals or from newborn rats. The extent of nerve fibre outgrowth was estimated by counting the number of nerve fibres crossing each arc of a sector drawn in the microscope(More)
  • T Lahtinen
  • 1989
Explants of hippocampus from rats at various ages evoked an intense nerve fibre growth from cocultured superior cervical ganglion and septum explants taken from newborn rats. The addition of antiserum to nerve growth factor (NGF) into the culture medium inhibited the outgrowth of nerve fibres from superior cervical ganglia, while septum explants still(More)
Sympathetic thoracic chain ganglia of 3-day-old rats were cultured in collagen gel medium for 24 hours together with explants from heart atrium, liver, kidney, cornea, iris, lung, adrenal cortex, adrenal medulla, skeletal muscle, or vas deferens. The extent of nerve fibre growth was estimated by counting the number of fibres crossing each arc of a sector(More)
Cytomegalovirus (CMV) has been demonstrated to induce class II antigen expression in endothelial cells. To study whether ganciclovir (DHPG) has an effect on CMV-induced class II expression, cultured rat heart endothelial cells were infected with rat CMV (RCMV) and treated with different DHPG concentrations. Class II antigens in endothelial cells were(More)