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Cell-free translation reconstituted with purified components
TLDR
A protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity was developed, and omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA). Expand
Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes.
TLDR
It is shown that the Hsc70/Hsp90 chaperone machinery is required to load small RNA duplexes into Argonaute proteins, but not for subsequent strand separation or target cleavage, and the data suggest that the chaper one machinery may serve as the driving force for the RISC assembly pathway. Expand
The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
TLDR
It is shown that tudor-domain containing 9 (Tdrd9) is essential for silencing Line-1 retrotransposon in the mouse male germline, and the results identify TDRD9 as a functional partner of MIWI2 and indicate that the tUDor-piwi association is a conserved feature. Expand
Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends.
TLDR
It is shown that piRNAs in Drosophila are 2'-O-methylated at their 3' ends, and Pimet mediates piRNA 2'- O-methylation in Dosophila. Expand
Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase*
TLDR
The results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylations by hPNPase, and polyadenyation is required for the stability of mt m RNAs. Expand
A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs
TLDR
In mammalian mitochondria, 22 species of tRNAs encoded in mitochondrial DNA play crucial roles in the translation of 13 essential subunits of the respiratory chain complexes involved in oxidative phosphorylation, and comprehensively determined the post-transcriptional modifications in each tRNA by mass spectrometry. Expand
Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease.
TLDR
It is concluded that the UUG codon-specific translational defect of the mutant mt tRNAs(Leu(UUR)) is the primary cause of MELAS at the molecular level. Expand
Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions.
TLDR
It is revealed that 2-thiouridine formation proceeds through a complex sulfur-relay system composed of multiple sulfur mediators that select and facilitate specific sulfur flow to 2- thiouridine from various pathways of sulfur trafficking. Expand
Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2.
TLDR
In livers from GLD-2-null mice, the steady-state level of the mature form of miR-122 was specifically lower than in heterozygous mice, whereas no reduction of pre-miR- 122 was observed, demonstrating that 3'-terminal adenylation by GLd-2 is required for the selective stabilization of mi R-122 in the liver. Expand
Mitochondria-specific RNA-modifying Enzymes Responsible for the Biosynthesis of the Wobble Base in Mitochondrial tRNAs
TLDR
The identification and characterization of a t RNA-modifying enzyme MTU1 (mitochondrial tRNA-specific 2-thiouridylase 1) that is responsible for the 2- thiolation of the wobble position in human and yeast mt tRNAs is described. Expand
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