PURPOSE
To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM).
METHODS
RT-PCR was used to examine the expression of… (More)
PURPOSE
To determine whether human conjunctival epithelium expresses any of the human mucin genes designated MUC2 through MUC7.
METHOD
Northern blot analysis was performed using total RNA isolated… (More)
PURPOSE
To determine the feasibility of using human amniotic membrane (AM) as a substrate for culturing oral epithelial cells and to investigate the possibility of using autologous cultivated oral… (More)
PURPOSE
To determine if human corneal and conjunctival epithelial synthesize MUC1 mucin, a membrane-spanning mucin present in a variety of simple epithelia.
METHODS
Reverse transcription-polymerase… (More)
PURPOSE
To investigate the outcome of cultivated corneal epithelial transplantation for severe stem cell deficiencies using denuded amniotic membrane (AM) as a carrier.
DESIGN
Retrospective,… (More)
PURPOSE
Surgery to reconstruct the ocular surface is greatly facilitated by the use of amniotic membrane, either as a biologic drape or, more recently, as a substrate for the transplantation of… (More)
PURPOSE
To evaluate the use of autologous serum (AS)-derived cultivated corneal epithelial transplantation for the treatment of severe ocular surface disease.
DESIGN
Retrospective noncomparative… (More)
OBJECTIVE
To evaluate the use of autologous serum (AS)-derived cultivated oral epithelial transplants for the treatment of severe ocular surface disease.
METHODS
We used AS from 10 patients with… (More)
PURPOSE
To examine the viability of using human amniotic membrane as substrate for culturing corneal epithelial cells and transplanting them onto severely injured rabbit eyes.
METHODS
An… (More)